棉酚对小鼠睾丸氧化损伤及NF-κB、P53和Caspase-3基因表达的影响  

Effects of gossypol on oxidative damage and expression of NF-κB,P53 and Caspase-3 in mice testis

作　　者：赵树臣[1,2] 王海涛 刘克祥[1,2] 侯振中[1,2] 赵立佳 ZHAO Shuchen;WANG Haitao;LIU Kexiang;HOU Zhenzhong;ZHAO Lijia(School of Veterinary Medicine,Northeast Agricultural University,Harbin 150030,China;Key Laboratory of the Provincial Education Department of Heilongjiang for Common Animal Disease Prevention and Treatment,Harbin 150030,China)

机构地区：[1]东北农业大学动物医学学院,哈尔滨150030 [2]黑龙江省普通高校动物普通疾病防治重点实验室,哈尔滨150030

出　　处：《东北农业大学学报》2024年第2期49-56,共8页Journal of Northeast Agricultural University

基　　金：国家重点研发项目子课题(2017YFD0502200)。

摘　　要：探究不同浓度棉酚引起小鼠睾丸组织氧化损伤和细胞凋亡的生殖毒性机理。将48只成年雄性小鼠随机分成4组,每日按0、40、80和120mg·kg-1剂量灌胃棉酚,连续20d。结果表明,灌胃棉酚后小鼠呈时间剂量依赖性中毒症状,与对照组(CG)相比较,中剂量组(MG)、高剂量组(HG)小鼠体重下降,MG组睾丸指数极显著降低(P<0.01)。小鼠睾丸组织抗氧化酶SOD、GSH-Px活性降低,睾丸组织中NF-κB(P65)mRNA表达水平,以及在组织总蛋白和核蛋白内蛋白表达水平降低,且呈剂量依赖性关系。睾丸组织内P53 mRNA和蛋白表达水平、Caspase-3mRNA和蛋白表达水平和MDA含量,三者均呈升高趋势,且呈剂量依赖性关系。提示棉酚染毒所致小鼠睾丸氧化应激损伤与细胞凋亡密切相关,可引起凋亡通路中P53和Caspase-3表达升高,NF-κB表达降低。In order to explore the effects of different gossypol concentrations on oxidative damage and the expression of NF-κB,P53 and Caspase-3 in mice testicular tissue,48 adult male mice were randomly divided into four groups,and were respectively been exposing to gossypol by oral according to 0,40,80 and 120 mg·kg-1·d-1 for 20 d.The results showed that the mice appeared obvious poisoning symptoms,the body weight in middle-dose group(MG)and high-dose group(HG)decreased as the time of exposure to gossypol lengthened.The testis indexes in MG significantly decreased(P<0.01)than those in control group(CG).The activities of SOD and GSH-Px significantly decreased.The NF-κB mRNA expression levels in testicular tissues and protein expression levels in total protein and nuclear protein significantly decreased in a dose-dependent manner.Conversely,the mRNA and protein expression levels of P53 and Caspase-3 in testicular tissues all increased dose-dependently as exposure to gossypol.The contents of MDA showed the same increase.It was suggested that oxidative damage induced by exposed gossypol was closely related to apoptosis in mice testis.It followed that reproductive toxicity was associated with enhancing the expression of P53 and Caspase-3 and inhibiting the expression of NF-κBin the apoptosis pathway.

关 键 词：棉酚 小鼠 睾丸 氧化应激 凋亡 

分 类 号：TQ645.97[化学工程—精细化工]