基于E2蛋白A-D抗原区检测猪瘟抗体间接ELISA方法的建立与应用  

Establishment and application of an indirect ELISA method for swine fever antibodiesbased on the A-D antigen region of E2 protein

作　　者：潘小慧 Pan Xiaohui(Jofunhwa Biotechnology(Fuzhou)Co.Ltd,Fuzhou350014)

机构地区：[1]兆丰华生物科技(福州)有限公司,福州350014

出　　处：《福建畜牧兽医》2024年第3期8-11,共4页Fujian Journal of Animal Husbandry and Veterinary medicine

摘　　要：本研究以猪瘟病毒E2蛋白主要抗原区E2 A-D为包被抗原,建立一种可特异性检测猪瘟(CSF)抗体的间接ELISA方法。该方法中抗原包被浓度为0.75μg/mL,检测血清稀释倍数为1:150。对123份兔体中和试验测定为阴性的样本进行临界值测定,确定OD450nm≥0.38为阳性,OD450nm≤0.31为阴性,0.31<OD450nm<0.38为可疑。对该方法的特异性、重复性和敏感性进行检验,同时与兔体中和试验结果进行平行比较,检测该方法的准确性。结果表明,本研究可为CSF血清抗体提供一种可替代、特异、稳定的血清学检测方法。In this study,the main antigen region E2 A-D of classical swine fever E2 protein was used as the coating antigen to establish an indirect ELISA antibody detection method for swine fever.The concentration of antigen coating was 0.75μg/mL and the dilution ratio of serum was 1:150.A critical value test was conducted on 123 serum samples that were negative in the rabbit neutralization test,and it was determined that OD450nm≥0.38 was positive,OD450nm≤0.31 was negative,and 0.31<OD450nm<0.38 was suspicious.The specificity,repeatability and sensitivity of the method were tested and the detection ability of this method was compared with the rabbit neutralization test.The results showed that this study could provide an alternative,specific and stable serological detection method for CSF antibody.

关 键 词：猪瘟病毒 间接ELISA E2蛋白 

分 类 号：S852.651[农业科学—基础兽医学]