微流控芯片制备载镁水凝胶核-壳微球的体外成骨活性研究  

Osteogenic activity of magnesium loaded hydrogel core-shell microspheres by microfluidic chip in vitro

作　　者：李龙 梁海 刘斌 吴健 朱洁 林正捷 LI Long;LIANG Hai;LIU Bin;WU Jian;ZHU Jie;LIN Zhengjie(Department of Stomatology,Shenzhen Qianhai Shekou Free Trade Zone Hospital,China,518067;State Key Laboratory of Oral&Maxillofacial Reconstruction and Regeneration,National Clinical Research Center for Oral Diseases,Shaanxi Key Laboratory of Stomatology,The Third Hospital Affiliated of Air Force Military Medical University,Xi'an;3D Printing Clinical Translational and Regenerative Medicine Center,Shenzhen Qianhai Shekou Free Trade Zone Hospital)

机构地区：[1]深圳市前海蛇口自贸区医院口腔科,518067 [2]口颌系统重建与再生全国重点实验室,国家口腔疾病临床医学研究中心,陕西省口腔医学重点实验室,空军军医大学第三附属医院口腔生物学教研室 [3]深圳市前海蛇口自贸区医院3D打印与再生医学创新中心

出　　处：《实用口腔医学杂志》2024年第3期351-359,共9页Journal of Practical Stomatology

基　　金：广东省深圳市南山区卫生科技项目(编号:NS2021064);深圳市科技计划资助(编号:JCYJ20220530151608019);广东省基础与应用基础研究项目(编号:2022A1515011815,2023A1515220232)。

摘　　要：目的:探索载镁水凝胶微球的在体外细胞实验中的成骨活性。方法:设计和搭载微流控芯片装置,并利用该装置载镁水凝胶核-壳微球精准调控镁离子在体外稳定释放,系统研究可控镁离子释放促进成骨活性机制。结果:微流控芯片制备出的载镁水凝胶微球(PLGA/MgO-alginate微球)呈现单分散性和特殊的核-壳结构调控镁离子在体外稳定释放的浓度稳定在50 ppm。不仅能显著增强MC3T3-E1前成骨细胞的活性和增殖能力,还可以改善其成骨分化和矿化能力,并增强了ALP活性。结论:载镁水凝胶核-壳微球能显著提高MC3T3-E1前成骨细胞的成骨活性。Objective:To explore the osteogenic activity of in vitro cultured preosteoblasts treated by magnesium loaded hydrogel core-shell microspheres.Methods:A microfluidics chip device was designed and made to fabricate magnesium loaded hydrogel core-shell microspheres to precisely control the stable release of magnesium ions in vitro,the mechanism of controllable magnesium ion release in the promotion of osteogenic activity of MC3T3-E1 preosteoblasts was systematically investigated.Results:The magnesium loaded hydrogel microspheres(PLGA/MgO-alginate microspheres)prepared by the microfluidics chip exhibited to be monodisperse with special core-shell structure and could control the stable release of magnesium ions at a concentration of 50 ppm in vitro.The in vitro study showed that it significantly enhanced the activity and proliferation ability of MC3T3-E1 preosteoblasts,promoted their osteogenic differentiation and mineralization ability,and increased ALP activity.Conclusion:Magnesium loaded hydrogel core-shell microspheres can promote the osteogenic activity of MC3T3-E1 osteoblasts in vitro.

关 键 词：微流控芯片 水凝胶 成骨活性 骨再生 镁离子 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]