不同氧疗方案对小鼠急性肾损伤保护作用的比较  

Comparison of the protective effects of different oxygen therapy regimens against acute kidney injury in mice

作　　者：徐峰 夏静 邵雅楠 李丹 吴新贺 XU Feng;XIA Jing;SHAO Yanan;LI Dan;WU Xinhe(Department of Nephrology,the Affiliated Rehabilitation Hospital of Nantong University,Jiangsu 226002;Department of Rehabilitation Medicine,the Affiliated Rehabilitation Hospital of Nantong University,Jiangsu 226002)

机构地区：[1]南通大学附属康复医院肾内科,江苏226002 [2]南通大学附属康复医院康复医学科,江苏226002

出　　处：《南通大学学报（医学版）》2024年第2期154-158,共5页Journal of Nantong University(Medical sciences)

基　　金：江苏省老年健康科研项目(LD2022009);南通市卫生健康委员会青年基金项目(QB2021009,QN2022034)。

摘　　要：目的:比较不同给氧方案对脂多糖(lipopolysaccharide,LPS)诱导的急性肾损伤(acute kidney injury,AKI)模型小鼠肾组织炎症性损伤及外周血炎症因子的影响。方法:采用ICR雌性小鼠,腹腔注射LPS(5 mg/kg)建立小鼠AKI模型,随机分成:(1)空白组(Control组),注射同等量的生理盐水;(2)模型组(LPS组);(3)纯氧治疗组(LPS+100%O_(2)组);(4)2.0绝对大气压(atmosphere absolute,ATA)高压氧(hyperbaric oxygen,HBO)治疗组(LPS+2.0 ATA HBO组);(5)3.0 ATA HBO治疗组(LPS+3.0 ATA HBO组),治疗后24 h,通过流式微球技术及实时荧光定量PCR(real-time PCR,RT-PCR)实验分别检测肾组织和外周血中促炎因子IL-1β、IL-6和TNF-α,以及抑炎因子IL-10水平。此外,通过HE染色法观察肾组织炎症性损伤的组织形态变化。结果:IL-1β、IL-6和TNF-α在LPS诱导24 h后均显著增加;100%O_(2)和2.0 ATA HBO治疗均可抑制促炎因子IL-1β、IL-6和TNF-α的释放,且2.0 ATA HBO抑制作用更显著(P<0.05或P<0.01);此外,LPS+2.0ATA HBO组抑炎因子IL-10水平显著增高(P<0.01)。HE染色结果表明,与LPS组相比,100%O_(2)和2.0 ATA HBO治疗后,肾组织炎症性损伤均有所减轻,且LPS+2.0 ATA HBO组改善效果最好(P<0.05或P<0.01),而LPS+3.0 ATA HBO组肾组织损伤未改善。结论:100%O_(2)和2.0 ATA HBO治疗均可明显改善AKI小鼠炎症性损伤,且2.0 ATA HBO改善作用更好。Objective:Comparison of the effects of different oxygen therapy regimens on renal tissue inflammatory injury and peripheral blood inflammatory factors in a mouse model of lipopolysaccharide(LPS)-induced acute kidney injury(AKI).Methods:ICR female mice were injected intraperitoneally with 5 mg/kg of LPS to establish a model of AKI.They were randomly divided into the following groups:(1)Control group(injected with the same amount of physiological saline);(2)Model group(LPS group);(3)Pure oxygen treatment group(injected with LPS and 100%O_(2));(4)2.0 atmosphere absolute(ATA)hyperbaric oxygen(HBO)treatment group(LPS+2.0 ATA HBO group);(5)3.0 ATA HBO treatment group LPS+3.0 ATA HBO group).The pro-inflammatory cytokines IL-1β,IL-6,and TNF-αand the anti-inflammatory cytokine IL-10 in peripheral blood and kidney tissue were detected by flow cytometric bead array and real-time PCR(RT-PCR)assays.The histomorphological changes of kidney tissue were observed by HE staining.Results:IL-1β,IL-6,and TNF-αlevels were significantly increased after 24 hours of LPS induction.100%O_(2)and 2.0 ATA HBO could inhibit the release of IL-1β,IL-6,and TNF-α,and 2.0 ATA HBO had a more pronounced impact,with a significantly increased IL-10 level in this treatment group.HE staining showed that compared with LPS,100%O_(2)and 2.0 ATA HBO could alleviate the inflammatory injury of kidney,and 2.0 ATA HBO showed a better effect.Nevertheless,3.0 ATA HBO did not reverse the damage to the kidney tissue.Conclusion:The above results suggested that both 100%O_(2)and 2.0 ATA HBO treatments significantly improved inflammatory injury in AKI mice.Moreover,2.0 ATA HBO had a superior effect on inhibiting tissue inflammation.

关 键 词：急性肾损伤 氧疗 高压氧 常压氧 炎症 细胞因子 小鼠 

分 类 号：R692[医药卫生—泌尿科学]