线粒体核糖体蛋白基因内含子间最佳匹配片段的相对位置分布  

The Relative Position Distributions of the Optimal Matched Segments Between Introns of Mitochondrial Ribosomal Protein Genes

作　　者：宋鑫伟 李瑞芳[1] 高姗 彭诗雅 SONG Xinwei;LI Ruifang;GAO Shan;PENG Shiya(College of Physics and Electronic Information,Inner Mongolia Normal University Hohhot 010022,China)

机构地区：[1]内蒙古师范大学物理与电子信息学院,内蒙古呼和浩特010022

出　　处：《内蒙古师范大学学报（自然科学版）》2024年第3期306-312,共7页Journal of Inner Mongolia Normal University(Natural Science Edition)

基　　金：内蒙古自治区自然科学基金资助项目“mRNA对蛋白质折叠速率影响机制的研究”(2023MS03019)。

摘　　要：对内含子间相互作用的研究是揭示基因表达调控作用的重要手段。为研究第一内含子与其他内含子间相互作用特征,并考虑内含子间的环状匹配特征包含了这种相互作用的重要信息,以人类、小鼠、红鳍东方鲀、黑腹果蝇和秀丽隐杆线虫五个物种的线粒体核糖体蛋白基因序列为样本,研究第一内含子与其他内含子间的环状匹配特征。采用Smith-Waterman方法,通过局部比对得到每个基因的第一内含子与其他内含子反向互补序列的最佳匹配片段,同时,将这些片段按8-mer CG模体的分类方式分为不同类型的片段。将基因组中所有第一内含子序列的长度标准化处理后,计算最佳匹配片段和各类片段CG对在第一内含子上的相对位置,并分析其物种差异性。结果显示,最佳匹配片段的相对位置分布没有显示出物种差异性,而其CG对的相对位置分布显示了物种差异性,且1CG类片段的CG对最可几位置随物种进化呈定向进化趋势。研究结果表明,这些最佳匹配片段可能是具有某些组织和功能的生物学元件,而CG模体体现了这些功能元件的重要特征。To investigate the interaction characteristics between the first intron and other introns and interpret the important information of the interaction contained in the circular matching feature between introns,the mitochondrial ribosomal protein gene sequences of five species,including Homo sapiens,Mus musculus,Fugu rubripes,Drosophila melanogaster and Caenorhabditis elegans,were used as samples to study the circular matching features between the first intron and other introns.Firstly,the Smith-Waterman algorithm was used to obtain the optimal matched segments of the reverse complementary sequences between the first intron and other introns of each gene through local alignment,and then these fragments were classified into different types based on the 8-mer CG motif.After standardization of sequence length of all first intron sequences in the genome,the relative position distribution of the optimal matched segments and CG pairs in different types of fragments on the first intron were calculated,and their species differences were analyzed.The results showed that the relative position distribution of the optimal matched segments did not present species differences,while the relative position distribution of CG pairs in different types of fragments presented species differences.Moreover,the most probable positions of CG pairs in the 1CG type fragments exhibited a directed evolutionary trend with species evolution.The result suggested that the optimal matched segments might be some kinds of organized and functional elements,and the CG motif reflected important characteristics of these functional elements.

关 键 词：内含子 最佳匹配片段 CG模体 相对位置分布 

分 类 号：Q61[生物学—生物物理学]