载ADSCs的GelMA水凝胶对糖尿病大鼠伤口愈合的作用  

Effect of ADSCs-loaded GelMA hydrogel on wound healing in diabetic rats

作　　者：王玥琪 赵志远 高菲[1] 项锋钢[1] 迟菁华[1] WANG Yueqi;ZHAO Zhiyuan;GAO Fei;XIANG Fenggang;CHI Jinghua(Department of Pathology,School of Basic Medicine,Qingdao University,Qingdao 266071,China)

机构地区：[1]青岛大学基础医学院病理学系,山东青岛266071 [2]青岛大学附属医院介入放射科,山东青岛266071

出　　处：《青岛大学学报（医学版）》2024年第2期199-204,共6页Journal of Qingdao University(Medical Sciences)

基　　金：教育部留学回国人员科研启动基金(80114020109)。

摘　　要：目的探究载脂肪间充质干细胞(ADSCs)的甲基丙烯酸酰化明胶(GelMA)水凝胶支架对糖尿病大鼠皮肤创伤愈合的影响。方法将ADSCs接种在GelMA支架表面。用链脲佐菌素构建糖尿病大鼠模型,在大鼠背部建立直径为2 cm的圆形全层皮肤缺损,将大鼠分为对照组(未行治疗)、支架组(单独应用支架治疗)及支架细胞组(应用载ADSCs支架治疗)。观察各组大鼠背部伤口愈合情况,采用苏木精-伊红染色对伤口皮肤组织进行组织学观察,采用免疫组织化学染色检测转化生长因子-β1(TGF-β1)表达。结果与对照组及支架组相比,支架细胞组在术后第14、21天创面愈合率更高,在术后第14天TGF-β1表达更强,在术后第21天毛细血管破裂出血减少。结论载ADSCs的GelMA水凝胶支架具有促进糖尿病大鼠伤口愈合的作用,TGF-β1可能在其中起重要作用。Objective To investigate the effect of adipose-derived stem cells(ADSCs)-loaded Gelatin Methacrylate(GelMA)hydrogel scaffolds on skin wound healing in diabetic rats.Methods ADSCs were seeded on the surface of GelMA scaffolds.Streptozocin was used to establish a rat model of diabetes,and a round full-thickness skin defect with a diameter of 2 cm was created on the back of the rats.The rats were divided into control group(without any treatment),GelMA group(treated with scaffolds alone),and ADSCs-loaded GelMA group(treated with ADSCs-loaded scaffolds).Wound healing was observed for each group;HE staining was used to observe the histology of skin tissue;immunohistochemical staining was used to measure the expression of transforming growth factor-beta 1(TGF-β1).Results Compared with the control group and the GelMA group,the ADSCs-loaded GelMA group had a higher wound healing rate at 14 and 21 days after surgery,a stronger expression of TGF-β1 at 14 days after surgery,and a reduction in capillary bleeding at 21 days after surgery.ConclusionADSCs-loaded GelMA hydrogel scaffolds can promote wound healing in diabetic rats,and TGF-β1 may play an important role in this process.

关 键 词：伤口愈合 糖尿病并发症 间质干细胞 水凝胶类 组织支架 病理学 大鼠 Sprague-Dawley 

分 类 号：R318.1[医药卫生—生物医学工程] R361.2[医药卫生—基础医学]