催化动力学荧光光谱法测定芹菜中铁的含量  

Catalytic-Kinetic Fluorescent Spectrometry for Detection of Iron(Ⅲ)in Celery

作　　者：宋少飞[1] 柴小川[1] 邓锦涛 周福林[1] SONG Shaofei;CHAI Xiaochuan;DENG Jintao;ZHOU Fulin(Department of Applied Chemistry,Yuncheng University,Yuncheng 044000,Shanxi,China)

机构地区：[1]运城学院应用化学系,山西运城044000

出　　处：《山西师范大学学报（自然科学版）》2024年第2期29-33,共5页Journal of Shanxi Normal University(Natural Science Edition)

基　　金：山西省高等学校科技创新项目(2019L0870);运城学院博士项目(YQ-2017030);运城学院学科建设经费资助项目(615174);运城市科技局软科学项目资助.

摘　　要：本文将荧光光度法与催化动力学相结合,在酸性介质中,铁(Ⅲ)对溴酸钾氧化二氯荧光素的反应具有很强的催化作用,以溴酸钾———二氯荧光素为反应体系,建立了一种测定痕量铁的催化动力学荧光新方法.对催化体系的最优反应条件进行了考察研究,在最优条件下,Fe(Ⅲ)浓度在1×10^(-7)~7×10^(-6)mg/mL范围内与荧光强度呈良好的线性关系,方法的检出限为6×10^(-8)mg/mL.对浓度为5×10^(-7)mg/mL的Fe(Ⅲ)标准溶液进行11次平行测定,相对标准偏差(RSD)为1.2%.该法简单、快速、灵敏,已用于蔬菜中铁含量的测定,回收率为90%~107.1%,结果令人满意.In this paper,catalytic kinetics and fluorescence spectrophotometry were combined.Based on the catalytic effect of iron(Ⅲ)on the oxidation of dichlorofluorescein by potassium bromate in acid solution,a new catalytic-kinetic fluorometry method for determination of trace iron was proposed with potassium bromate-di-chlorofluorescein as the reaction system.The optimum conditions affecting the catalytic reaction have been inves-tigated.Under the optimal conditions,the iron(Ⅲ)concentration within a range of 1×10^(-7)~7×10^(-6)mg/mL has a good linear relation,and the detection limit is 6×10^(-8)mg/mL.The relative standard deviation(RSD)is 1.2%for 5×10^(-7)mg/mL iron(Ⅲ)solution(n=11).The method has the advantages of simplicity and rapid-ity,and has been applied for the detection of iron(Ⅲ)in vegetables.The recoveries between 90%~107.1%are obtained with satisfied results.

关 键 词：催化动力学 二氯荧光素 荧光光谱法 铁 

分 类 号：O657.32[理学—分析化学]