木珊瑚科Cladopsammia gracilis和Rhizopsammia wettsteini线粒体全基因组比较与系统进化分析  

Complete mitochondrial genome of Cladopsammia gracilis and Rhizopsammia wettsteini(Scleractinia,Dendrophylliidae)and its phylogenetic implications

作　　者：霍嘉欣 李颖心 宋严 朱晴 周伟华[1,2,3] 袁翔城 黄晖[1,2,3] 刘胜 HUO Jiaxin;LI Yingxin;SONG Yan;ZHU Qing;ZHOU Weihua;YUAN Xiangcheng;HUANG Hui;LIU Sheng(CAS Key Laboratory of Tropical Marine Bio-resources and Ecology,Guangdong Provincial Key Laboratory of Applied Marine Biology,South China Sea Institute of Oceanology,Chinese Academy of Sciences,Guangzhou 510301,China;CAS-HKUST Sanya Joint Laboratory of Marine Science Research,Key Laboratory of Tropical Marine Biotechnology of Hainan Province,Sanya 572000,China;Sanya National Marine Ecosystem Research Station,Tropical Marine Biological Research Station in Hainan,Chinese Academy of Sciences,Sanya 572000,China;University of Chinese Academy of Sciences,Beijing 100049,China)

机构地区：[1]中国科学院热带海洋生物资源与生态重点实验室,广东省应用海洋生物学重点实验室,中国科学院南海海洋研究所,广东广州510301 [2]三亚海洋科学综合(联合)实验室,海南省热带海洋生物技术重点实验室,海南三亚572000 [3]海南三亚海洋生态系统国家野外科学观测研究站,中国科学院海南热带海洋生物实验站,海南三亚572000 [4]中国科学院大学,北京100049

出　　处：《热带海洋学报》2024年第3期22-30,共9页Journal of Tropical Oceanography

基　　金：广东省基础与应用基础研究基金(2022A1515010656);海南省重点研发计划项目(ZDYF2020200);国家重点研发项目(2021YFF0502800)。

摘　　要：木珊瑚科(Dendrophylliidae)下的枝沙珊瑚属(Cladopsammia)和Rhizopsammia属的物种分类目前仍存在模糊的界定,本研究结合形态特征与微观结构和线粒体全基因组测序对Cladopsammia gracilis与Rhizopsammia wettsteini进行形态和分子进化水平的分析比较。结果显示:形态学特征上,R.wettsteini通过生殖根出芽,珊瑚肋上具有小尖状突起,隔片薄而脆;而C.gracilis则无生殖根,珊瑚肋上突起为细瘤状,隔片颗粒感强且较厚。两个物种的线粒体基因组组成十分相似,同样包含13个蛋白编码基因、2个rRNA基因和2个tRNA基因,与大部分六放珊瑚相同;在基因重叠情况和密码子偏好性上则有别于大部分六放珊瑚。基于CO1基因的系统进化分析显示,C.gracilis和R.wettsteini关系较近,却与枝沙珊瑚属(Cladopsammia)其他物种关系较远,因此C.gracilis是否归入枝沙珊瑚属(Cladopsammia)值得探讨,同时提示我们需要重新审视枝沙珊瑚属(Cladopsammia)的分类地位。There is a taxonomic uncertainty on the species classification of Cladopsammia gracilis and Rhizopsammia wettsteini well as the unresolved status of the closely related genera Cladopsammia and Rhizopsammia.In order to further analyze the phylogenetic relationship between C.gracilis and R.wettsteini,we examined the micromorphological and microstructural features and systematically compared the gene structure,genetic distance and codon preference of two mitochondrial genomes.The results show that there are three distinct characteristics:reptoid budding,costae and septa.The genome composition of the two species is very similar,containing 13 protein-coding genes,2 rRNA genes and 2 tRNA genes.Unlike most scleractinians,there were no overlaps between genes.In addition,phylogenetic analysis based on CO1 genes show that C.gracilis and R.wettsteini are closely related but distantly related to other species of the genera Cladopsammia.The present study highlights the need for an integrative approach to conduct a deeper species delimitation of genera Cladopsammia and Rhizopsammia.

关 键 词：木珊瑚科 线粒体基因组 分类学 进化分析 

分 类 号：Q958.8[生物学—动物学]