牛大力多糖的荧光标记、鉴定及其体内分布  

Fluorescence labeling,identification,and in vivo distribution of Millettia speciosa polysaccharide

作　　者：多水银 张兴波 吴玉辉 李静涛 杨雨辉 DUO Shui-yin;ZHANG Xing-bo;WU Yu-hui;LI Jing-tao;YANG Yu-hui

机构地区：[1]海南大学热带农林学院,海南海口570100

出　　处：《饲料研究》2024年第8期88-91,共4页Feed Research

基　　金：国家自然科学基金项目(项目编号:32260900);海南省自然科学基金高层次人才项目(项目编号:321RC481)。

摘　　要：研究旨在探究牛大力多糖在小鼠体内的吸收与分布。使用罗丹明B标记牛大力多糖得到牛大力多糖罗丹明B荧光标记物(MSP-RhB)。使用荧光显微镜和红外光谱扫描鉴定标记成功后,给小鼠灌胃MSP-RhB,分别于1、3、6、12、24h取材,通过小动物活体成像仪系统观察MSP-RhB在小鼠体内、体外的分布情况。结果显示:荧光标记牛大力多糖成功;灌胃小鼠后,MSP-RhB主要分布于胃肠道、肝脏和肾脏;MSP-RhB在1h到达小肠各段,约3h达到最大值,在小肠中停留时间长达24h。研究表明,MSP-RhB通过小肠吸收,先进入全身循环,然后分布到肝脏和肾脏中,且荧光信号随时间的推移逐渐减弱。The study was to explore the absorption and distribution of Millettia speciosa polysaccharide in mice.The Millettia speciosa polysaccharide Rhodamine B fluorescent marker(MSP-Rh B)was obtained by labeling Millettia speciosa polysaccharide with Rhodamine B.After the labels were identified by fluorescence microscope and infrared spectrum scanning,MSP-Rh B was given to mice by intragastric administration.Samples were collected at 1,3,6,12,24 h,respectively,and the distribution of MSP-Rh B invivoand invitrowas observed by small animal invivoimaging system.The results showed that fluorescence labeling of Millettia speciosa polysaccharide was successful.After intragastric administration,MSP-Rh B was mainly distributed in gastrointestinal tract,liver and kidney.MSP-Rh B reached each segment of the small intestine at 1 h,reached its maximum at about 3 h,and stayed in the small intestine for 24 h.Studies have shown that MSP-Rh B is absorbed through the small intestine,enters the systemic circulation first,and then distributes to the liver and kidney,and the fluorescence signal gradually decreases with time.

关 键 词：牛大力多糖 荧光标记 口服吸收 体内分布 

分 类 号：S816.7[农业科学—饲料科学]