无花果叶发酵条件优化及其对活性成分含量的影响  

Optimization of fermentation conditions of fig leaves and its effect on content of active ingredients

作　　者：董华恒 陈营[1] DONG Hua-heng;CHEN Ying

机构地区：[1]烟台大学生命科学学院,山东烟台264005

出　　处：《饲料研究》2024年第8期97-102,共6页Feed Research

基　　金：烟台市重点研发计划(项目编号:2018ZDCX018)。

摘　　要：研究利用菌酶协同技术发酵无花果叶,以期提高无花果叶中活性成分的析出率。以无花果叶为唯一碳源配制培养基,从新鲜、冻存及晒干的无花果叶中均筛选出嗜麦芽寡养单胞菌,该菌发酵无花果叶产香豆素的含量高于未发酵组,故选定其作为发酵菌种。发酵2 d后,通过比色法检测发酵液中香豆素和萜类化合物的含量,通过单因素试验和正交试验优化培养条件。结果显示,优化后最优发酵方式为菌酶协同发酵,最佳前体物为8 mg/L的苯丙氨酸,额外碳源为2 g/L的麦芽糖,最佳氮源为3 g/L的硫酸铵+蛋白胨混合物,纤维素酶和果胶酶的最佳比例为1:2。优化培养后无花果叶发酵液中香豆素、萜类化合物、黄酮类化合物以及多糖含量分别提高了92.66%、59.40%、44.68%和42.84%。优化后多酚类化合物含量是优化前的4.24倍。研究表明,无花果叶发酵可以提高其活性成分的含量,研究可为无花果叶作为一种新型饲料添加剂的开发利用提供依据。In order to improve the extraction rate of active components in fig leaves,the fermentation of fig leaves by bacterial enzyme was studied.A strain of Stenotrophomonas maltophilia was isolated from fresh,frozen,and dried fig leaves using fig leaves as the sole carbon source.It was found that the coumarin content of fermented fig leaves was higher than that of unfermented fig leaves.Therefore,this bacterium strain was selected as the fermentation strain in this experiment.After fermentation for two days,the coumarin and terpenes contents in the fermentation broth were determined by colorimetry,and the culture conditions were optimized by single factor and orthogonal experiments.The results showed that the optimal fermentation mode was bacterial-enzyme co-fermentation,the optimal precursor was 8 mg/L of L-phenylalanine,the additional carbon source was 2 g/L maltose,the optimum nitrogen source was ammonium sulfate+peptone mixture of 3 g/L,and the optimum ratio of cellulase and pectinase was 1∶2.At the optimization condition,the contents of coumarins,terpenoids,flavonoids,and polysaccharides in the fermentation broth of fig leaves were increased by 92.66%,59.40%,44.68%,and 42.84%respectively.The content of polyphenols was 4.24 times higher than that before optimization.The study indicates that fig leaves fermentation can increase the content of its active components,and the study provides a basis for the development and utilization of fig leaves as a new feed additive.

关 键 词：无花果叶 嗜麦芽寡养单胞菌 优化培养 活性成分 

分 类 号：S816.7[农业科学—饲料科学]