活性维生素D对高糖条件下肾小管上皮细胞肾素的影响  

Effect of active vitamin D on renin in renal tubular epithelial cells under high glucose conditions

作　　者：何一晴 廖文文 陶密 黎煜妍 高苹[1] He Yi-qing;Liao Wen-wen;Tao Mi;Li Yu-yan;Gao Ping(Division of Nephrology,Zhongnan Hospital of Wuhan University,Wuhan 430071,China)

机构地区：[1]湖北省武汉市武汉大学中南医院肾内科,武汉430071

出　　处：《临床肾脏病杂志》2024年第5期399-405,共7页Journal Of Clinical Nephrology

基　　金：湖北省卫生健康委员会(WJ2021M165)。

摘　　要：目的研究活性维生素D对高糖条件下大鼠肾小管上皮细胞(NRK-52E)肾素、(前)肾素受体[(Pro)renin receptor,PRR]、核因子κB(nuclear factor kappa-B,NF-κB)的影响及NF-κB在其中所起的作用。方法将体外培养的NRK-52E细胞分为7组,分别命名为对照组、高糖组、高糖+PDTC(吡咯烷二硫代氨基甲酸铵)组、高糖+0.1/1.0/10.0/100.0 nM帕立骨化醇组。分别用实时荧光定量PCR和蛋白质印迹检测各组肾素、PRR、NF-κB p65的mRNA和蛋白表达,用染色质免疫沉淀-定量PCR检测NF-κB p65与肾素基因启动子的关系。结果与对照组相比,高糖组肾素、PRR、NF-κB的表达均有增加(P<0.05)。与高糖组相比,高糖+PDTC组肾素和NF-κB的表达下降(P<0.05),高糖+100.0 nM帕立骨化醇组肾素、PRR、NF-κB的表达均下调(P<0.05),染色质免疫沉淀-定量PCR证明NF-κB直接与肾素的基因启动子区域结合。结论活性维生素D可能通过抑制NF-κB减少高糖诱导的肾小管上皮细胞肾素的表达。Objective To explore the effect of active vitamin D on renin,(pro)renin receptor(PRR)and NF-κB in rat renal tubular epithelial(NRK-52E)cells in high glucose conditions and elucidate the role of NF-κB.Methods NRK-52E cells were cultured in vitro and divided into 7 groups of control,high glucose,high glucose+PDTC(pyrrolidinedithiocarbamate ammonium)and high glucose+0.1/1/10/100 nM paricalcitol.mRNA and protein expression of renin,PRR and NF-κB p65 in each group were detected by quantitative polymerase chain reaction(qPCR)and Western blot.ChIP-qPCR was uti-lized for detecting the relationship between NF-κB p65 and the promoter of renin.Results The expressions of renin,PRR and NF-κB spiked in high-glucose group as compared with control group(P<0.05).The ex-pressions of renin and NF-κB declined in high-glucose+PDTC group as compared with high-glucose group(P<0.05).And the expressions of renin,PRR and NF-κB became down-regulated in high-glucose+100 nM paricalcitol group(P<0.05).ChIP-qPCR demonstrated that NF-κB directly conjugated to the gene promoter region of renin.Conclusion Active vitamin D may reduce high glucose-induced renin expression in renal tubular epithelial cells through an inhibition of NF-κB.

关 键 词：糖尿病肾脏病 活性维生素D 肾素 (前)肾素受体 核因子ΚB 

分 类 号：R587.2[医药卫生—内分泌] R692.9[医药卫生—内科学]