Immune response to inactivated bacterial vector carrying the recombinant K39 antigen of Leishmania infantum in mice  

作　　者：Lucelina S.Araújo Bruno B.Silva Eduarda N.F.N.Santos Arnaldo S.Bezerra Samuel S.Frota Assis R.Montenegro Eridan O.P.T.Florean Maurício Fvan Tilburg Maria Izabel F.Guedes 

机构地区：[1]Laboratory of Biotechnology and Molecular Biology,Northeast Biotechnology Network,State University of Ceará,Fortaleza,Brazil [2]Faculty of Veterinary Medicine,State University of Ceará,Fortaleza,Brazil

出　　处：《Asian Pacific Journal of Tropical Biomedicine》2024年第5期199-206,共8页亚太热带生物医学杂志（英文版）

基　　金：supported by grants from the Brazilian Agencies:Coordenação de Aperfeiçoamento de Pessoal de Nível Superior(CAPES-Financial code 001);Conselho Nacional de Desenvolvimento Científico e Tecnológico(CNPq);Fundação Cearense de Apoio ao Desenvolvimento Científico e Tecnológico(FUNCAP).

摘　　要：Objective:To evaluate the immunological response elicited by an inactivated bacterial vector carrying the K39 antigen of Leishmania infantum,and a purified antigen.Methods:Mice were subjected to the following treatments:(1)Purified recombinant K39(rK39)protein at a 20μg dose with complete Freund’s adjuvant;(2)Inactivated Escherichia coli(BL21 DE3)carrying the K39 protein at an equivalent total protein content of 200μg;(3)Inactivated bacteria lacking the K39 protein;(4)Non-immunized control animals.Serological monitoring was performed.All groups were challenged by intraperitoneal injection of 10^(7) Leishmania infantum promastigotes.After euthanasia,the liver and spleen were collected to analyze the levels of TNF,IFN-γ,IL-12,IL-4,and IL-10.Results:Mice immunized with purified rK39 or the inactivated bacterial vector carrying the K39 antigen of Leishmania infantum showed a long-lasting immune response with high levels of polyclonal antibodies specifically recognizing the recombinant proteins.The IgG1 subclass was the predominant immunoglobulin;however,the induction of IgG2a and the profile of cytokines produced were indicative of the induction of a mixed-type response.Conclusions:The inactivated bacterial vector carrying the K39 antigen,as well as the purified antigen can induce a long-lasting immune response in immunized mice,predominantly favouring a Th2 profile response.

关 键 词：Visceral leishmaniasis K39 Inactivated bacterial vector Vaccine Immune response Th1 TH2 Leishmania infantum 

分 类 号：R757.9[医药卫生—皮肤病学与性病学]