绿豆GPX基因家族鉴定及其对非生物胁迫的响应  

Identification of the GPX gene family in Vigna radiata and its response to abiotic stress

作　　者：邱涛涛 苏鸿飞 王瑜 张禾佳 胡慧欣 阮诗婷 袁星星[2] 谢彦杰[1] QIU Taotao;SU Hongfei;WANG Yu;ZHANG Hejia;HU Huixin;RUAN Shiting;YUAN Xingxing;XIE Yanjie(College of Life Sciences,Nanjing Agricultural University,Nanjing 210095,China;Institute of Cash Crops,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China)

机构地区：[1]南京农业大学生命科学学院,江苏南京210095 [2]江苏省农业科学院经济作物研究所,江苏南京210014

出　　处：《南京农业大学学报》2024年第3期453-461,共9页Journal of Nanjing Agricultural University

基　　金：江苏省基础研究计划杰出青年基金项目(BK20220084);江苏省基础研究计划青年基金项目(BK20200282)。

摘　　要：[目的]本文利用生物信息学方法从绿豆基因组中筛选谷胱甘肽过氧化物酶(glutathione peroxidase,GPX)基因家族成员,并探究其组织表达模式及其对若干胁迫的响应。[方法]利用生物信息学的方法对绿豆GPX家族的等电点、相对分子质量、共线性、上游顺式作用元件等进行分析,通过转录组测序和RT-qPCR进一步分析绿豆GPX基因家族的组织表达模式以及在不同逆境胁迫和激素作用下的表达水平。[结果]从绿豆基因组中筛选出8个GPX基因,其不均匀分布于1/2/3/5/10号染色体上。基因结构及基序分析结果显示,8个GPX的基因结构类似,均含有6个内含子区域,并且大部分GPX成员都具有UTR区。转录组以及RT-qPCR分析结果表明,多个GPX家族成员均能响应多种逆境胁迫。组织表达分析结果显示,不同GPX家族成员在不同组织中呈特异性表达;GPX家族整体在绿豆中表达丰度较低,但表达量能被各种胁迫强烈诱导。[结论]绿豆GPX基因家族响应多种非生物胁迫,不同GPX成员响应的胁迫类型不同。[Objectives]This research used bioinformatics methods to screen and identify the GPX gene family in Vigna radiata(mung bean),and to explore its expression pattern and function in abiotic stress.[Methods]The isoelectric point,molecular weight,collinearity and cis-acting elements of the GPX gene family in mung bean were analyzed by bioinformatics methods.The expression patterns of the GPX gene family in mung bean and its expression levels under different abiotic stresses,and hormones were further analyzed by transcriptome data.Transcriptome sequencing and RT-qPCR were used to further analyze the tissue expression patterns and the expression levels of GPX gene family in V.radiata under different stress and hormones.[Results]Total of 8 GPX genes were identified in mung bean genome,which were unevenly distributed on chromosome 1/2/3/5/10.According to the gene structure and motif analysis,the eight GPX genes had similar structures,all containing six intronic regions,and most of the GPX members had UTR regions.Transcriptome and RT-qPCR analysis showed that multiple GPX members were able to respond to a variety of stress.Based on the expression of GPX in different tissues,different GPX members were specifically expressed in different tissues.GPX expression in mung bean was low and might be strongly induced under stress.[Conclusions]The GPX gene family of mung bean responded to a variety of abiotic stresses,and different GPX members responded to different types of stress.

关 键 词：绿豆 GPX基因家族 非生物胁迫 基因表达 

分 类 号：Q946.2[生物学—植物学]