吸入氢气对高氧诱导新生SD大鼠支气管肺发育不良(BPD)的影响  

Effects of hydrogen gas inhalation on hyperoxia⁃induced bronchopulmonary dysplasia in neonatal SD rats

作　　者：张兰 李敏许[2] 周福心[2] 黄为民 ZHANG Lan;LI Minxu;ZHOU Fuxin;HUANG Weimin(Department of Neonatology,the First Clinical Medical College of Southern Medical University(Nanfang Hospital),Guangzhou,Guangdong,China,510515;Department of Neonatology,Dongguan Maternal And Child Health Care Hospital,Dongguan,Guangdong,China,523000)

机构地区：[1]南方医科大学第一临床医学院(南方医院)新生儿科,广东广州510515 [2]东莞市妇幼保健院新生儿科,广东东莞523000

出　　处：《分子诊断与治疗杂志》2024年第5期909-912,共4页Journal of Molecular Diagnostics and Therapy

基　　金：东莞市社会科技发展(重点)项目(201750725007076)。

摘　　要：目的探讨吸入氢气对高氧诱导新生SD大鼠支气管肺发育不良(BPD)的影响。方法选取新生SD大鼠吸入85%高氧建立支气管肺发育不良模型。分为control组(正常空气)9只、BPD组(85%O_(2))13只、H_(2)组(2%H_(2))12只及Treat组(85%O_(2)+2%H_(2))16只,共4组。记录各组大鼠体重;14 d后取肺组织,采用苏木精-伊红(HE)染色法进行病理切片观察与评价;肺组织匀浆上清液检测肺损伤的标志物神经酰胺(Cer);取血,检测脑损伤标志物肌钙结合蛋白(S100B)、心肌损伤标志物心肌肌钙蛋白(cTnI)并进行比较。结果BPD组体重增长显著慢于control组,差异有统计学意义(F=7.122,<0.05)。HE染色结果显示高氧使肺泡简化,肺泡数量明显减少,吸入氢气可有效改善;4组肺泡平均截距比较,BPD组>Treat组肺泡>control组与H_(2)组,差异有统计学意义(F=24.951,P<0.05)。Cer水平BPD组>control组、H_(2)组与Treat组,cTnI水平BPD组>H_(2)组>Treat组>control组,S100B水平BPD组>Treat组>H_(2)组、control组,差异具有统计学意义(F=19.361、26.321、13.235,P<0.05)。结论吸入H_(2)可改善肺损伤,改善脑损伤与心肌损伤,其机制可能与抗氧化有关。Objective To investigate the effect of hydrogen inhalation on bronchopulmonary dys⁃plasia(BPD)in hyperoxy⁃induced neonatal SD rats.Methods The bronchopulmonary dysplasia model was established by exposing neonatal SD rats to of 85%hyperoxygen through inhalation.The rats were divided into foue groups:the control group(normal air)9,the BPD group(85%O_(2))13,the H_(2) group(2%H_(2))12 and the treat group(85%O_(2)+2%H_(2))16.The body weight of each group was recorded.After 14 days,the rats were sacrificed,and blood and lung tissues were collected.Pathological sections were observed and evaluated using hematoxylin⁃eosin(HE)staining.The supernatant of lung tissue homogenate was used to detect the lung injury marker ceramide(Cer).Blood samples were taken to detect the brain injury marker tropont⁃binding pro⁃tein(S100B),and the myocardial injury marker myocardial troponin(cTnI).The results were then compared among the groups.Results The body weight increase in the BPD group was significantly slower than that in the control group(F=7.122,P<0.05).HE staining results showed that hyperoxia simplified the alveoli and sig⁃nificantly reduced the number of alveoli,which could be effectively improved by inhaling hydrogen gas.The average intercept of alveoli in the BPD group was greater than that in the treat group and the control group,and this difference was significantly different from the H_(2) group(F=24.951,P<0.05).The Cer level in the BPD group was higher than that in the control group,the H_(2) group and the treat group.The cTnI level in the BPD group was higher than that in the H_(2) group,the treat group and the control group.The S100B level in the BPD group was higher than that in the treat group,the H_(2) group,and the control group.These differences were statistically significant(F=19.361,26.321,13.235,P<0.05).Conclusion Inhaling H_(2) can improve lung injury,brain injury and myocardial injury.The mechanism may be related to antioxidants.

关 键 词：氢气 支气管肺发育不良 大鼠 高氧 

分 类 号：R722.6[医药卫生—儿科]