DNATyper^(TM)21与Verifiler^(TM)Plus试剂盒对常规案件检材检验的比较  

Comparative Evaluation of DNATyper^(TM)21 and Verifi ler^(TM)Plus Kit in Forensic DNA Casework

作　　者：王剑[1] 马云龙[1] 余政梁 龚政 高馨玉 杨亮 张丽娜 WANG Jian;MA Yunlong;YU Zhengliang;GONG Zheng;GAO Xinyu;YANG Liang;ZHANG Lina(Heilongjiang Provincial Public Security Department,Harbin 150008,China;Institute of Forensic Science,Ministry of Public Security,Beijing 100038,China;Harbin Public Security Bureau,Harbin 150010,China;Qiqihar Public Security Bureau,Qiqihar 161005,Heilongjiang,China;Mudanjiang Public Security Bureau,Mudanjiang 157399,Heilongjiang,China;Harbin Railway Public Security Bureau,Harbin150001,China)

机构地区：[1]黑龙江省公安厅,哈尔滨150008 [2]公安部鉴定中心,北京100038 [3]哈尔滨市公安局,哈尔滨150010 [4]齐齐哈尔市公安局,黑龙江齐齐哈尔161005 [5]牡丹江市公安局,黑龙江牡丹江157399 [6]哈尔滨铁路公安局,哈尔滨150001

出　　处：《刑事技术》2024年第3期318-322,共5页Forensic Science and Technology

基　　金：法医遗传学公安部重点实验室开放课题基金资助项目(2020FGKFKT03)。

摘　　要：本文通过比较DNATyper^(TM)21与Verifi ler^(TM)Plus两种STR检测试剂盒对各类案件检材的检验能力,探讨DNATyper^(TM)21试剂盒在常规案件检验中应用的可靠性。取0.03125、0.0625、0.125、0.25、0.5、1.0 ng/μL基因组标准品对两种试剂盒进行灵敏度测试;应用DNATyper^(TM)21与Verifi ler^(TM)Plus试剂盒分别对1056例常规案件检材DNA进行检验;对同一样本等位基因进行一致性比较。结果表明,两种试剂盒均可以成功检测出模板浓度在0.0625 ng/μL以上的标准DNA。在检验的1056例样本中,DNATyper^(TM)21试剂盒共检出881例,检出率为83.4%;Verifi ler^(TM)Plus试剂盒检出892例,检出率为84.5%。统计学结果表明两种试剂盒检出率无统计学差异,且同一样本相同基因座得到的等位基因分型一致。综上,DNATyper^(TM)21试剂盒对各类常规检材具有良好的检验能力,可应用于日常案件检验。The DNATyper^(TM)21 amplification kit is a 5-dye multiplex assay,which is widely used in construction of the Chinese National autosomal DNA database.However,the DNATyper^(TM)21 kit has not been extensively used in forensic DNA casework.Therefore,this study aimed to elucidate the reliability of the DNATyper^(TM)21 kit compared with Verifi ler^(TM)Plus kit and to assess its applicability on forensic analysis.Standard DNA samples with concentrations of 0.03125,0.0625,0.125,0.25,0.5 and 1.0 ng/μL were used for sensitivity testing.DNATyper^(TM)21 kit and Verifi ler^(TM)Plus kit were utilized to detect the DNA extracted from 1056 casework evidence samples and the allele consistency of the same sample was analyzed.The results showed that both kits could successfully detect standard DNA with a template concentration above 0.0625 ng/μL.For casework evidence samples,DNATyper^(TM)21 kit successfully detected 881 cases out of 1056,and the detection rate is 83.4%,while Verifiler^(TM)Plus kit successfully detected 892 cases out of 1056,and the detection rate is 84.5%.The statistical results showed that there was no significant difference in the detection rate between the two kits,and the alleles obtained at the same locus of the same sample were completely consistent.In conclusion,the DNATyper^(TM)21 is considered to have the capability to be used for STR analysis of casework evidence samples.

关 键 词：法医物证学 DNATyper^(TM)21试剂盒 Verifi ler^(TM)Plus试剂盒 比较研究 生物检材 

分 类 号：D918.9[政治法律—法学]