还原氧化石墨烯/金纳米颗粒修饰电极在新冠病毒检测中的应用  

Reduced Graphene Oxide/Gold Nanoparticles Decorated Electrodes for the Detection of SARS-Cov-2

作　　者：李亚婷 刘仲明 杨欢 张海燕 Li Yating;Liu Zhongming;Yang Huan;Zhang Haiyan(Guangzhou University of Chinese Medicine,Guangzhou,510006;General Hospital of Southern Theatre Command,Guangzhou,510010)

机构地区：[1]广州中医药大学,广州510006 [2]南部战区总医院,广州510010

出　　处：《化学通报》2024年第5期620-624,共5页Chemistry

摘　　要：使用电化学沉积法在丝网印刷碳电极表面制备了还原氧化石墨烯和金纳米颗粒,构建了一种用于新冠病毒检测的石墨烯电化学传感器。通过扫描电镜和相应的电化学方法对纳米复合材料在电极表面的成功修饰进行了表征分析。采用差分脉冲伏安法对传感器的性能进行检测,实验构建的电化学传感器具有良好的灵敏度,最低检测限为7.09×10^(-9)mol/L,线性范围为10^(-10)~10^(-6)mol/L,并具有良好的灵敏度和特异性,可以在未进行核酸扩增的情况下直接检测新冠病毒临床样本。The reduced graphene oxide and gold nanoparticles were prepared by electrochemical deposition on the surface of screen-printed carbon electrodes to construct a graphene electrochemical sensor for SARS-Cov-2 detection.The successful modification of the nanocomposites on the electrode surface was characterized and analyzed by SEM and corresponding electrochemical methods.The performance of the sensor was also tested by differential pulse voltammetry,and the experimentally constructed electrochemical sensor has good sensitivity,with a minimum detection limit of 7.09×10^(-9)mol/L,a linear range of 10^(-10)~10^(-6)mol/L,and good sensitivity and specificity for the direct detection of SARS-Cov-2 clinical samples without nucleic acid amplification.

关 键 词：还原氧化石墨烯 纳米金 电化学传感器 丝网印刷碳电极 新冠病毒 

分 类 号：O657.1[理学—分析化学] R318[理学—化学]