蛋白质分子机器的结构动态的多尺度多方法研究  

An integrative approach to understand structural dynamics of protein molecular machines across spatiotemporal scales

作　　者：朱月玲 龚洲 唐淳[2] Yueling Zhu;Zhou Gong;Chun Tang(Innovation Academy for Precision Measurement Science and Technology Chinese Academy of Sciences,Wuhan 430071,China;College of Chemistry and Molecular Engineering,Peking University,Beijing 100871,China;University of Chinese Academy of Sciences,Beijing 100049,China)

机构地区：[1]中国科学院精密测量科学与技术创新研究院,武汉430071 [2]北京大学学化学与分子工程学院,北京100871 [3]中国科学院大学,北京100049

出　　处：《中国科学：化学》2024年第5期788-801,共14页SCIENTIA SINICA Chimica

基　　金：国家重点研发计划重点专项(编号:2018YFA0507700)资助项目。

摘　　要：蛋白质构成了生物体内最重要的分子机器,对蛋白质分子机器动态特性的了解、运作机制的揭示有助于实现对其功能的调控.本文对蛋白质分子机器结构动态的研究方法进行了全面而系统的综述,从空间分辨率、时间尺度、离体与原位等角度,对不同方法的特点和应用进行了深入的分析,指出了它们的优势和不足以及多方法整合联用的策略.文章还对未来蛋白质分子机器结构动态研究的发展方向进行了展望,提出了前瞻性的观点.Proteins constitute the most important molecular machines within biological organisms.Understanding the dynamic properties of protein molecular machines and revealing their working mechanisms are crucial for the regulation of their functions.This article provides a comprehensive and systematic review of the research methods for the structural dynamics of protein molecular machines.It delves into the characteristics and applications of different methods from perspectives such as spatial resolution,time-scale,in vitro versus in vivo.Additionally,the review highlights the advantages and limitations of individual methods as well as strategies for an integrated approach.It also looks forward to the future development on the structural dynamics of protein molecular machines and provides authoritative viewpoints.

关 键 词：蛋白质机器 动态结构 多方法整合 时空尺度 

分 类 号：O641.3[理学—物理化学] O629.73[理学—化学]