Phylogenetic, phylogeographic and divergence time analysis of Anopheles subpictus species complex using ITS2 and COI sequences  

作　　者：Lihini Sandaleka Muthukumarana Methsala Madurangi Wedage Samanthika Rathnayake Nissanka Kolitha De Silva 

机构地区：[1]Department of Zoology,Faculty of Applied Sciences,University of Sri Jayewardenepura,Gangodawila,Nugegoda,Sri Lanka [2]Genetics and Molecular Biology Unit,Faculty of Applied Sciences,University of Sri Jayewardenepura,Gangodawila,Nugegoda,Sri Lanka [3]Sri Lanka Institute of Biotechnology,Pitipana,Homagama,Sri Lanka

出　　处：《Asian Pacific Journal of Tropical Medicine》2024年第5期214-225,I0004-I0038,共47页亚太热带医药杂志（英文版）

摘　　要：Objective:To address the phylogenetic and phylogeographic relationship between different lineages of Anopheles(An.)subpictus species complex in most parts of the Asian continent by maximum utilization of Internal Transcriber Spacer 2(ITS2)and cytochrome C oxidase I(COI)sequences deposited at the GenBank.Methods:Seventy-five ITS2,210 COI and 26 concatenated sequences available in the NCBI database were used.Phylogenetic analysis was performed using Bayesian likelihood trees,whereas median-joining haplotype networks and time-scale divergence trees were generated for phylogeographic analysis.Genetic diversity indices and genetic differentiation were also calculated.Results:Two genetically divergent molecular forms of An.subpictus species complex corresponding to sibling species A and B are established.Species A evolved around 37-82 million years ago in Sri Lanka,India,and the Netherlands,and species B evolved around 22-79 million years ago in Sri Lanka,India,and Myanmar.Vietnam,Thailand,and Cambodia have two molecular forms:one is phylogenetically similar to species B.Other forms differ from species A and B and evolved recently in the above mentioned countries,Indonesia and the Philippines.Genetic subdivision among Sri Lanka,India,and the Netherlands is almost absent.A substantial genetic differentiation was obtained for some populations due to isolation by large geographical distances.Genetic diversity indices reveal the presence of a long-established stable mosquito population,at mutation-drift equilibrium,regardless of population fluctuations.Conclusions:An.subpictus species complex consists of more than two genetically divergent molecular forms.Species A is highly divergent from the rest.Sri Lanka and India contain only species A and B.

关 键 词：Molecular systematics ITS2 COI DNA sequences Phylogeny PHYLOGEOGRAPHY 

分 类 号：R531.3[医药卫生—内科学] R181.3[医药卫生—临床医学]