独脚金内酯及其抑制剂对红花石蒜小鳞茎发育的影响  

Effects of Strigolactone and Its Inhibitor on Bulblets Development of Lycoris radiata

作　　者：赵小琼 姜燕婷 杨昆 王奕 张志坤 陈沫先 李克虎 ZHAO Xiaoqiong;JIANG Yanting;YANG Kun;WANG Yi;ZHANG Zhikun;CHEN Moxian;LI Kehu(Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region(Ministry of Education),College of Life Sciences,Guizhou University,Guiyang,Guizhou 550025;National Key Laboratory of Green Pesticide,Research and Development Center of Fine Chemicals,Guizhou University,Guiyang,Guizhou 550025,China)

机构地区：[1]贵州大学生命科学学院,山地植物资源保护与种质创新教育部重点实验室,贵州贵阳550025 [2]贵州大学精细化工研究开发中心,绿色农药全国重点实验室,贵州贵阳550025

出　　处：《贵州农业科学》2024年第5期24-29,共6页Guizhou Agricultural Sciences

基　　金：贵州省科学技术厅基础研究计划项目(黔科合基础-ZK〔2023〕一般123)。

摘　　要：【目的】探明独脚金内酯(SL)及独脚金内酯抑制剂对石蒜小鳞茎发育的影响,为石蒜类植物繁育方法提供参考。【方法】以红花石蒜为材料,分别用1μmol/L、5μmol/L、10μmol/L的人工合成独脚金内酯(rac-GR24)及其抑制剂(Tis108)对基盘切割后的种球进行浸泡处理,通过分析比较小鳞茎发育过程的形态学特征和处理后60 d小鳞茎的发生数量和重量,明确独脚金内酯对红花石蒜小鳞茎发育的影响。【结果】rac-GR24和Tis108处理对红花石蒜小鳞茎的发生数量和膨大速度有不同影响,其中,1μmol/L rac-GR24处理的小鳞茎发生数量最多,繁殖系数达5.31;10μmol/L Tis108处理的小鳞茎发生数量最少,繁殖系数只有2.76;1μmol/L Tis108处理的小鳞茎膨大速度最快,处理60 d后新生小鳞茎平均重量达0.133 g,1μmol/L、5μmol/L和10μmol/L rac-GR24处理的小鳞茎膨大速度与CK(清水处理)无显著差异;10μmol/L Tis108处理的小鳞茎膨大速度最慢,处理60 d后新生小鳞茎平均重量达0.026 g。【结论】独脚金内酯及其抑制剂低浓度处理有利于促进红花石蒜小鳞茎的发生,独脚金内酯抑制剂低浓度处理能显著促进红花石蒜小鳞茎增重。【Objective】The effects of strigolactone(SL)and its inhibitor on the bulblets development of Lycoris radiata were explored,which provided reference for breeding method for Lycoris plants.【Method】The L.radiata bulblets were cut and soaked with different concentrations(1μmol/L,5μmol/L,10μmol/L)of synthesized strigolactone(rac-GR24)and its inhibitor(Tis108).Through analyzing and comparing the morphology characteristics of the development process of small bulblets and the formation quantity and weight of bulblets after 60 days of treatment,the effects of strigolactone on bulblets development of L.radiata were determined.【Result】The rac-GR24 and Tis108 demonstrated different effects on the formation quantity and swelling speed of L.radiata bulblets.1μmol/L rac-GR24 treatment induced the largest number of bulblets,and the reproduction coefficient reached 5.31.10μmol/L Tis108 treatment resulted in the smallest number of bulblets,and the propagation coefficient was only 2.76.1μmol/L Tis108 treatment led to the fastest bulblet swelling speed,and the average weight of new bulblets was 0.133 g at 60 days after treatment.There was no significant difference in bulblet swelling speed between 1μmol/L,5μmol/L,10μmol/L rac-GR24 and CK.10μmol/L Tis108 treatment had the slowest bulblet swelling speed,and the average weight of new bulblets was 0.026 g after 60 days of treatment.【Conclusion】The treatment of strigolactone and its inhibitor with low concentration is beneficial to the formation of L.radiata bulblets.The inhibitor for strigolactone with low concentration can significantly promote the weight gain of L.radiata bulblets.

关 键 词：红花石蒜 小鳞茎 生长发育 独脚金内酯 独脚金内酯抑制剂 

分 类 号：S682.2[农业科学—观赏园艺]