芸苔素内酯浓度对干旱胁迫大白菜幼苗生长及生理指标的影响  

Effects of Brassinolide Concentrations on Growth and Physiological Indexes of Chinese Cabbage Seedlings Under Drought Stress

作　　者：张新梅 赵文麟 和芬 许彬[2] 许俊强[1,2] 张国平 ZHANG Xinmei;ZHAO Wenlin;HE Fen;XU bin;XU Junqiang;ZHANG Guoping(Dian-Tai Engineering Research Center for Characteristic Agriculture Industrialization of Yunnan Province,Yunnan Agricultural University,Kunming,Yunnan 650201;Key Laboratory of Vegetable Biology of Yunnan Province,College of Horticulture a and Landscape,Yunnan Agricultural University,Kunming,Yunnan 650201,China)

机构地区：[1]云南农业大学云南省滇台特色农业产业化工程研究中心,云南昆明650201 [2]云南农业大学园林园艺学院/云南省蔬菜生物学重点实验室,云南昆明650201

出　　处：《贵州农业科学》2024年第5期41-46,共6页Guizhou Agricultural Sciences

基　　金：云南省农业联合专项“类钙调蛋白CML8参与调控黄瓜性型分化功能解析”(202101BD070001-115);云南省基础研究项目“黄瓜类钙调蛋白CML8参与Ca2+缓解幼苗盐胁迫机制研究”(202201AT070502);云南省重大科技计划项目“设施蔬菜标准化生产关键技术研究与应用”(202102AE090005);云南省科技人才和平台计划“周艳虹专家工作站”(202205AF150017);云南省教育厅科学研究基金项目“外源Ca2+及其抑制剂对盐胁迫下黄瓜幼苗发育的影响”(2023Y0987)。

摘　　要：【目的】探明不同浓度芸苔素内酯对干旱胁迫大白菜幼苗抗旱性的调控作用,为芸苔素内酯在大白菜生产中的应用提供参考。【方法】以大白菜胜丽118为试验材料,苗期喷施不同浓度(0.05 mg/L、0.1 mg/L、0.5 mg/L、1 mg/L)28-表高芸苔素内酯后,选用10%浓度的聚二乙醇-6000溶液模拟干旱胁迫,测定大白菜生长和生理生化指标,分析不同浓度芸苔素内酯处理大白菜幼苗生理活性差异。【结果】随芸苔素内酯浓度升高,大白菜幼苗长势呈先良好后衰败变化;从大白菜幼苗表型看,芸苔素内酯浓度0.05 mg/L、0.1 mg/L处理的耐旱效果最明显。干旱胁迫下叶片POD和CAT活性随芸苔素内酯浓度升高呈先增强后减弱趋势,MDA含量随浓度升高呈先降后升趋势。芸苔素内酯浓度过高大白菜幼苗叶绿素相对含量变低。【结论】喷施适宜浓度的芸苔素内酯可提高干旱胁迫下大白菜幼苗的抗氧化酶活性,有效缓解干旱胁迫造成的损害,适宜的芸苔素内酯浓度为0.05~0.1 mg/L。【Objective】The regulation effects of brassinolide with different concentrations on drought resistance of Chinese cabbage seedlings under drought stress were studied,so as to provide reference for applying brassinolide during production of Chinese cabbage.【Method】The Chinese cabbage‘Shengli 118’was selected as the test material,and polyethylene glycol-6000 solution with 10%concentration was selected to simulate drought stress.After spraying different concentrations(0.05 mg/L,0.1 mg/L,0.5 mg/L and 1 mg/L)of 28-homobrassinolide,the growth and physiochemical indexes were determined to analyze the differences in physiological activity of Chinese cabbage seedlings treated with different concentrations of brassinolide.【Result】With the increase of brassinolide concentration,the growth state of cabbage seedlings showed a trend of good at first and then decayed.Form the aspect of phenotype of Chinese cabbage,their drought tolerance was the most obvious when treated with 0.05 mg/L and 0.1 mg/L brassinolide.The POD and CAT activities of leaves under drought stress showed a tendency to enhance and then weaken with brassinolide concentration increasing,while the MDA content decreased and then increased.The high concentration of brassinolide resulted in lower relative chlorophyll content of Chinese cabbage seedlings.【Conclusion】Spraying appropriate concentrations of brassinolide can improve the antioxidant enzyme activities of cabbage seedlings under drought stress,effectively alleviate the damage caused by drought stress,and spraying brassinolide at 0.05 mg/L and 0.1 mg/L concentrations are the best.

关 键 词：大白菜 幼苗 芸苔素内酯 干旱胁迫 酶活性 

分 类 号：S634.1[农业科学—蔬菜学]