硫酸羟氯喹治疗狼疮性肾炎过程中肾小球内蛋白质表达谱的变化  

Changes in the protein expression profiles of glomeruli during the treatment of lupus nephritis with hydroxychloroquine sulfate

作　　者：李艳秋[1] 王海潮 郑晓杰 刘晓丹[1] 刘雪 田淑艳 姚丽[1] LI Yanqiu;WANG Haichao;ZHENG Xiaojie;LIU Xiaodan;LIU Xue;TIAN Shuyan;YAO Li(Department of Nephrology,The First Hospital of China Medical University,Shenyang 110001,China)

机构地区：[1]中国医科大学附属第一医院肾脏内科,沈阳110001

出　　处：《中国医科大学学报》2024年第5期401-404,413,共5页Journal of China Medical University

基　　金：国家自然科学基金(81273107);沈阳市科技计划(211-173-9-67)。

摘　　要：目的筛选硫酸羟氯喹(HCQ)在治疗狼疮性肾炎(LN)过程中肾小球内差异表达的蛋白。方法60只雌性NZB/WF1小鼠饲养至28周龄时,选择蛋白尿3+~4+的40只小鼠,将其分为HCQ组和对照组。饲养至36周时,经胸主动脉灌注磁珠分离肾小球蛋白,应用双向荧光差异凝胶电泳(2D-DIGE)和基质辅助激光解吸电离飞行时间质谱(MALDI-TOF-MS)分析和鉴定差异表达蛋白,应用免疫组织化学染色验证候选蛋白在2组小鼠肾小球中的表达。结果HCQ组和对照组相比共筛选出31个差异表达蛋白,包括24个上调蛋白和7个下调蛋白。结论HCQ治疗LN过程中RI和ENOA等蛋白在肾小球内表达差异明显,这些蛋白可能参与LN肾小球病变的发生和发展,是HCQ治疗LN的潜在靶点。Objective To screen the differentially expressed proteins in glomeruli during the treatment of lupus nephritis(LN)with hydroxychloroquine sulfate(HCQ).Methods Sixty female NZB/WF1 mice were used.At the age of 28 weeks,40 mice with proteinuria of 3+to 4+were divided into HCQ and control groups.After feeding for 36 weeks,the glomerular proteins were extracted by magnetic beads and analyzed by two-dimensional fluorescence difference gel electrophoresis(2D-DIGE)and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry(MALDI-TOF-MS).The expression of the candidate proteins in the glomeruli of the LN mice was examined by immunohistochemistry.Results A total of 31 differentially expressed proteins were identified between the two groups,including 24 upregulated and seven downregulated proteins.Conclusion The expression of proteins like RI and ENOA in the glomeruli of LN mice was significantly different during HCQ treatment.These proteins may be involved in the pathogenesis of LN and are therefore potential targets of HCQ in the treatment of LN.

关 键 词：肾小球 狼疮性肾炎 蛋白质组学 双向电泳 

分 类 号：R692.3[医药卫生—泌尿科学]