高氏盆炎方二号方对慢性盆腔炎大鼠的抗炎抗粘连作用及对ICAM-1和caspase-8表达的影响  

Anti-inflammatory and anti-adhesion effects of the No.2 Gao's Penyan prescription in rats with chronic pelvic inflammatory disease and its influences on the expression of ICAM-1 and caspase-8

作　　者：张晴 高慧[2] 郝艳红 刘玉兰[2] 侯金萌 林静[2] 刘振华[2] ZHANG Qing;GAO Hui;HAO Yanhong;LIU Yulan;HOU Jinmeng;LIN Jing;LIU Zhenhua(Traditional Chinese Medicine College,North China University of Science and Technology,Tangshan 063000;Affiliated Hospital of Chengde Medical University,Chengde 067000,China)

机构地区：[1]华北理工大学中医学院,河北唐山063000 [2]承德医学院附属医院,河北承德067000

出　　处：《临床医学研究与实践》2024年第16期1-7,共7页Clinical Research and Practice

基　　金：国家中医药管理局2014年全国名老中医药专家高慧传承工作室建设项目(No.2015048157);河北省中医药重点学科(中医妇科学)建设项目(No.202200505334);河北省中医药管理局2018年度中医药类科研计划课题(No.2018164);河北省中医药管理局2017年度中医药类科研计划课题(No.2017082)。

摘　　要：目的探讨高氏盆炎方二号方对慢性盆腔炎(CPID)大鼠的抗炎抗粘连作用及对细胞间黏附分子-1(ICAM-1)和半胱天冬酶-8(caspase-8)表达的影响。方法首先通过体外抑菌试验观察高氏盆炎方二号方药液的最低抑菌浓度(MIC)。将75只雌性SD大鼠随机分为空白组(n=10)和造模组(n=65)。造模10 d后,在造模组随机抽取5只大鼠处死,进行子宫组织HE染色后镜下呈慢性炎症改变,提示造模成功。随后将60只大鼠再随机分为模型组、中药组、中成药组及人胎盘组织液组,其中中药组又分为低、中、高剂量组,每组10只。模型组给予生理盐水2 mL/只灌胃,中药低、中、高剂量组分别给予高氏盆炎方二号方7、14、28 g/kg灌胃,中成药组给予蒲苓盆炎康颗粒3 g/kg灌胃,人胎盘组织液组给予人胎盘组织液1 mL/kg腹腔注射。以上各给药组均每日1次给药,连续给药20 d。末次给药24 h后处死大鼠并取子宫组织称重,计算子宫肿胀度及子宫系数;光镜下观察大鼠子宫组织病理学改变情况;用免疫组化法及Western-blot法检测大鼠子宫组织中ICAM-1和caspase-8蛋白表达水平。结果高氏盆炎方二号方对金黄色葡萄球菌、大肠杆菌及乙型溶血性链球菌有明显的抑菌作用。与空白组比较,模型组的子宫肿胀度升高,子宫系数降低(P<0.05),提示模型建立成功;与模型组比较,各给药组的子宫肿胀度降低(P<0.05),尤以中药组高、中剂量组及人胎盘组织液组改善显著。与模型组比较,各给药组大鼠子宫组织中的ICAM-1蛋白表达水平降低,caspase-8蛋白表达水平升高(P<0.05),提示给药治疗有效;空白组与中药高剂量组、中药中剂量组及人胎盘组织液组的ICAM-1、caspase-8蛋白表达水平比较,差异无统计学意义(P>0.05),说明以上三组疗效相当。结论高氏盆炎方二号方具有抑菌的作用,能够有效改善疾病症状、控制宫腔粘连的发展,修复受损的子宫组织形态�Objective To investigate the anti-inflammatory and anti-adhesion effects of the No.2 Gao's Penyan prescription in rats with chronic pelvic inflammatory disease(CPID)and its influences on the expression of intercellular cell adhesion molecule-1(ICAM-1)and caspase-8.Methods Firstly,the minimum inhibitory concentration(MIC)of the No.2 Gao's Penyan prescription was observed by in vitro antibacterial test.Seventy-five female SD rats were randomly divided into blank group(n=10)and modeling group(n=65).After 10 d of modeling,5 rats were randomly selected from the modeling group and sacrificed,after HE staining of uterine tissue,chronic inflammatory changes were observed under the microscope,suggesting that the modeling was successful.Subsequently,60 rats were randomly divided into model group,Chinese medicine group,Chinese patent medicine group and human placental tissue fluid group,the rats of the Chinese medicine group were divided into low,medium and high dose groups,with 10 rats in each group.The model group was given 2 mL of normal saline by gavage,the low,medium and high dose groups of Chinese medicine were given 7,14 and 28 g/kg of the No.2 Gao's Penyan prescription,the Chinese patent medicine group was given 3 g/kg of Puling Penyankang granules by gavage,the human placenta tissue fluid group was given 1 mL/kg of human placenta tissue fluid by intraperitoneal injection.Each of the above administration groups was administered once a day for 20 consecutive days.The rats were sacrificed 24 h after the last administration,and the uterine tissue was weighed,the uterine swelling degree and uterine coefficient were calculated;the pathological changes of rat uterus were observed under light microscope;the expression levels of ICAM-1 and caspase-8 protein in rat uterine tissue were detected by immunohistochemistry and Western-blot.Results The No.2 Gao's Penyan prescription had obvious antibacterial effect on Staphylococcus aureus,Escherichia coli and beta hemolytic Streptococcus.Compared with the blank group,the uterine s

关 键 词：慢性盆腔炎 高氏盆炎方二号方 细胞间黏附分子-1 半胱天冬酶-8 

分 类 号：R271.13[医药卫生—中医妇科学]