黄花苜蓿MfMYB3R-3基因克隆及结构与功能的生物信息学分析  

Cloning of MfMYB3R-3 Gene of Medicago falcata L.and Bioinformatics Analysis of Structure and Function

作　　者：柴华 杨曌 申忠宝[3] 李莎莎 王晓龙 徐艳霞 吴玥 CHAI Hua;YANG Zhao;SHEN Zhongbao;LI Shasha;WANG Xiaolong;XU Yanxia;WU Yue(Heilongjiang Academy of Agricultural Sciences Postdoctoral Programme,Harbin 150086,China;Branch of Animal Husbandry and Veterinary of Heilongjiang Academy of Agricultural Sciences,Qiqihar Heilongjiang 161005,China;Institute of Forage and Grassland Science,Heilongjiang Academy of Agricultural Science,Harbin 150086,China)

机构地区：[1]黑龙江省农业科学院博士后科研工作站,哈尔滨150086 [2]黑龙江省农业科学院畜牧兽医分院,黑龙江齐齐哈尔161005 [3]黑龙江省农业科学院草业研究所,哈尔滨150086

出　　处：《种子》2024年第5期33-39,共7页Seed

基　　金：黑龙江省博士后面上项目(LBH-Z22259);黑龙江省农业科学院农业科技基础创新项目(CX23YQ10)。

摘　　要：MYB转录因子是植物中分布最广的转录因子家族之一,在植物响应生物及非生物胁迫,调控逆境胁迫相关基因表达方面起着重要的作用。本研究基于黄花苜蓿转录组数据,鉴定和克隆了黄花苜蓿MfMYB3R-3基因,并进行了生物信息学分析。结果表明,MfMYB3R-3基因CDS区长1701 bp,编码566个氨基酸,具有SANT保守结构域,为MYB3R型转录因子;MfMYB3R-3蛋白化学分子式为C_(2690)H_(4314)N_(796)O_(873)S_(20),理论等电点为8.75,该蛋白是亲水性不稳定偏碱性非分泌蛋白,不具有信号肽,无跨膜结构,亚细胞定位预测在细胞核。系统进化分析发现,黄花苜蓿MfMYB3R-3蛋白与蒺藜苜蓿的MYB3R-3的亲缘关系最近。MYB transcription factor is one of the most widely distributed transcription factor families in plants,and plays an important role in regulating the expression of stress-related genes in response to biological and abiotic stresses.In this study,based on the transcriptome data of Medicago falcata L.,MfMYB3R-3 gene was identified and cloned,and the bioinformatics was analyzed.The results showed that coding sequence of MfMYB3R-3 was 1701 bp long,with 566 amino acids encoded and SANT preserving structure domain,which was the MYB3R type transcription factor.MfMYB3R-3 protein chemical formula was C_(2690)H_(4314)N_(796)O_(873)S_(20),theoretical isoelectric point was 8.75,this protein was hydrophilic unstable alkaline non-secretory protein,no signal peptide,no transmembrane structure,subcellular localization predicted in the nucleus.The phylogenetic analysis showed that MfMYB3R-3 protein of Medicago falcata L.was closely related to MYB3R-3 of Medicago truncatula.

关 键 词：黄花苜蓿 MfMYB3R-3基因 克隆 生物信息学 

分 类 号：Q781[生物学—分子生物学]