基于SSR分子标记的紫苏种质资源遗传多样性及群体结构分析  

Genetic Diversity and Population Structure Analysis of the Perilla frutescens L.Germplasm Resources Based on SSR Markers

作　　者：向依 潘金卫 李慧琳 奉斌 杨航 袁婷婷 于二汝 XIANG Yi;PAN Jinwei;LI Huilin;FENG Bin;YANG Hang;YUAN Tingting;YU Erru(Institute of Oil Crops,Guizhou Academy of Agricultural Sciences,Guiyang 550009,China)

机构地区：[1]贵州省农业科学院油料研究所,贵阳550009

出　　处：《种子》2024年第5期40-47,F0002,共9页Seed

基　　金：贵州省科技支撑项目“贵州山区特色油料资源利用研究及实验平台建设”[黔科中引地(2020)4012号];贵州省农科院青年基金项目“贵州紫苏优异种质资源发掘以及遗传多样性分析”[黔农科院青年基金(2021)05号];“紫苏资源核心种质及其SSR指纹图谱的研究”(黔农科青年基金(2023)13号);“紫苏油加工及副产品综合利用的关键技术研究与示范”(黔科合支撑[2021]一般183)。

摘　　要：为了明确不同紫苏种质的遗传基础和群体的亲缘关系,本研究利用19对SSR分子标记对不同地理来源的141份紫苏资源进行遗传多样性和群体结构分析。结果表明,19对SSR分子标记扩增出80条多态性条带,平均每对引物4.26条,多态性指数(PIC)变幅在0.3311~0.8457之间,平均为0.6206,多态性条带占比85.10%。利用UPGMA法对141份紫苏进行分子聚类,遗传相似系数(GS)介于0.4787~0.9489之间,平均为0.7099,在GS=0.696处将供试材料分为三类。通过Structure群体结构分析,将供试材料分为三大类,其中68.8%的紫苏种质资源遗传背景比较单一,31.2%的资源拥有混合血统,其遗传背景较为复杂。In order to clarify the genetic basis of different Perilla frutescens L.germplasm and population relationship,19 pairs of SSR molecular markers were used to analyze the genetic diversity and population structure of 141 Perilla frutescens L.resources from different geographical sources.The results showed that 80 polymorphic bands were amplified from 19 pairs of SSR markers,with an average of 4.26 bands per primer pair.The polymorphism index(PIC)ranged from 0.3311 to 0.8457,with an average of 0.6206 bands,accounting for 85.10%.Molecular clustering of 141 Perilla was performed by UPGMA method,and the genetic similarity coefficient(GS)ranged from 0.4787 to 0.9489,with an average of 0.7099.The materials were divided into three classes at GS=0.696.Through the analysis of Structure and population structure,the tested materials were divided into 3 groups.Among them,68.8%of Perilla frutescens L.germplasm resources had a single genetic background,31.2%had mixed ancestry,and their genetic background was more complex.

关 键 词：紫苏 遗传多样性 SSR标记 群体结构分析 

分 类 号：S565.9[农业科学—作物学]