基于ISSR和TRAP分子标记的东北地区栽培黑木耳遗传多样性研究  

Genetic Diversity Analysis of Cultivated Auricularia heimuer Based on ISSR and TRAP Molecular Markers

作　　者：焦枥禾 张丕奇[1] 王凤利 张秀丰 岳欣 戴肖东[1] JIAO Lihe;ZHANG Piqi;WANG Fengli;ZHANG Xiufeng;YUE Xin;DAI Xiaodong(Institute of Microbiology,Heilongjiang Academy of Sciences,Harbin 150010,China;Heilongjiang Huateng Biotechnology Co.,Ltd.,Jiamusi Heilongjiang 154000,China)

机构地区：[1]黑龙江省科学院微生物研究所,哈尔滨150010 [2]黑龙江华腾生物科技有限公司,黑龙江佳木斯154000

出　　处：《种子》2024年第5期112-118,共7页Seed

基　　金：黑龙江省“揭榜挂帅”科技攻关项目(2022ZXJ06B04)。

摘　　要：为探究东北地区黑木耳栽培菌株的遗传多样性及亲缘关系,采用ISSR和TRAP分子标记对50株栽培黑木耳菌株进行遗传多样性分析。利用22条高多态性、高分辨率的ISSR和TRAP分子标记引物,对栽培黑木耳进行了DNA扩增。结果表明,黑木耳ISSR和TRAP标记谱带多态性较高,分别占89.58%和84.71%,遗传相似系数为0.53~1.00;在ISSR标记的聚类图中相似系数大于0.96的菌株占44.0%,TRAP标记的聚类图中相似系数大于0.96的菌株占54.0%;STRUCTURE聚类结果显示,50株菌株可分为四大类;遗传多样性分析结果表明,东北地区黑木耳栽培菌株的遗传多样性主要来源于群体间,黑木耳栽培菌株的遗传多样性欠丰富,生产菌种资源的同质化较为突出。In order to explore the genetic diversity and genetic relationship of cultivated Auricularia heimuer strains in northeast China,genetic diversity of 50 cultivated Auricularia heimuer strains was analyzed by ISSR and TRAP molecular markers.The DNA of cultivated Auricularia heimuer was amplified by 22 highly polymorphic and high-resolution ISSR and TRAP labeled primers.The results showed that the polymorphisms of ISSR and TRAP markers were high,accounting for 89.58%and 84.71%,respectively,with genetic similarity coefficients ranging from 0.53 to 1.00.The strains with similarity coefficient greater than 0.96 accounted for 44.0%in ISSR labeled cluster plots,and 54.0%in TRAP labeled cluster plots.The STRUCTURE clustering results showed that the 50 strains could be divided into four categories.The results of genetic diversity analysis showed that the genetic diversity of Auricularia heimuer cultivated strains in northeast China was mainly from inter-population,and the genetic diversity of Auricularia heimuer cultivated strains was not rich,and the homogeneity of production strain resources was prominent.

关 键 词：黑木耳 遗传多样性 分子标记 ISSR TRAP 

分 类 号：S646.6[农业科学—蔬菜学]