基于F_(2∶3)家系的玉米籽粒相关性状QTL定位与候选基因分析  

QTL Mapping and Candidate Gene Analysis of Kernel Related Traits by Using Maize F_(2∶3) Family Lines

作　　者：何玥 聂蕾 郭爽 王栋 涂亮 刘鹏飞 郭向阳[2] 王安贵[2] 祝云芳[2] 吴迅 陈泽辉[2] HE Yue;NIE Lei;GUO Shuang;WANG Dong;TU Liang;LIU Pengfei;GUO Xiangyang;WANG Angui;ZHU Yunfang;WU Xun;CHEN Zehui(Agricultural College of Guizhou University,Guiyang 550025,China;Institute of Upland Food Crops,Guizhou Academy of Agricultural Sciences,Guiyang 550006,China;Ministry Agriculture and Rural Affairs Key Laboratory of Crop Genetic Resources and Germplasm Innovation in Karst Region,Guiyang 550006,China)

机构地区：[1]贵州大学农学院,贵阳550025 [2]贵州省农业科学院旱粮研究所,贵阳550006 [3]农业农村部喀斯特山区作物基因资源与种质创新重点实验室,贵阳550006

出　　处：《种子》2024年第5期119-124,156,共7页Seed

基　　金：贵州省科技支撑计划(黔科合支撑[2022]重点026);贵州省科技支撑计划(黔科合支撑[2022]重点029);贵州省科技支撑计划项目(黔科合支撑[2022]重点025);国家自然科学基金项目(32171981、32060460、32160451);黔农科院国基后补助[2021]16号、[2021]55号、[2022]02号和[2022]09号;贵州省玉米现代农业产业技术体系遗传育种功能实验室(GZCYTX2023-0102);贵州省农业科学院科技创新项目([2022]03号);贵州省育种科研基础平台创新能力建设(黔科合服企[2022]014);贵州省自然科学基金项目(黔科合基础[2022]一般236)和十层次人才项目([2018]5629)。

摘　　要：本研究以热带玉米骨干自交系T32和QR273构建的F_(2∶3)家系为材料,运用高通量GBS测序技术对其进行基因型鉴定,结合该群体在两个不同环境下的籽粒相关性状评价结果,利用完备区间作图法,共定位到52个控制玉米籽粒相关性状的QTL,其中粒长相关QTL 17个,粒宽相关QTL 24个,百粒重相关QTL 11个,可解释1.44%~14.48%的表型变异。其中3个主效QTL分别位于第1、2、10号染色体上,贡献率分别为14.48%、12.00%、10.21%。利用生物信息学分析,筛选出控制籽粒相关性状的候选基因3个,分别是Zm00001d02010、Zm00001d023914、Zm00001d020418,通过参与和调控植物体内各项生理活动和信号传导从而影响籽粒的大小。In this study,F_(2∶3) families constructed from tropical maize backbone inbred lines T32 and QR273 were identified by high-throughput GBS sequencing technology,and the results of grain-related traits evaluation in two different environments were combined.A total of 52 QTLs controlling grain related traits were identified by complete interval mapping,including 17 QTLs related to grain length,24 QTLs related to grain width,and 11 QTLs related to 100 grain weight,which could explain 1.44%-14.48%of phenotypic variation.Three major QTLs were located on chromosome 1,2 and 10,with contribution rates of 14.48%,12.00%and 10.21%,respectively.Three candidate genes controlling grain-related traits,Zm00001d02010,Zm00001d023914 and Zm00001d020418,were screened by bioinformatics analysis,which affected the grain size by participating in and regulating various physiological activities and signal transduction in plants.

关 键 词：玉米 籽粒相关性状 QTL 候选基因 

分 类 号：S513[农业科学—作物学]