Study on the regulatory effect of liver X receptor in HEK293 cells by six main diterpene esters in Semen Euphorbiae  

作　　者：Si-Yuan Ma Fan-Miao Kong Xiao-Tong Wei Jun-Li Zhang Hai-Ting Zhu Xin-Ning Zhang Yu-Feng Hu Ming-Rui Jiang Hui-Nan Wang Yi-Cen Xu Ying-Zi Wang 

机构地区：[1]School of Chinese Materia Medica,Beijing University of Chinese Medicine,Beijing 102488,China

出　　处：《TMR Modern Herbal Medicine》2024年第2期35-40,共6页TMR现代中药

基　　金：supported by National Natural Science Foundation of China(Grant No.82074021).

摘　　要：Background:To study the effects of the main diterpene esters in Euphorbia factor L_(1),L_(2),L_(3),L_(7a),L_(7b)and L_(8)on the transcriptional activity and protein expression of liver X receptor(LXR).Methods:The effect of the main diterpene ester components in Semen Euphorbiae on the viability of HEK293 cells were studied by MTT assay.The LXR-Luc plasmid vector was transfected into HEK293 cells and treated with Euphorbia factor L_(1),L_(2),L_(3),L_(7a),L_(7b)and L_(8)for 24 h.The effect of the main diterpene ester components of Semen Euphorbiae on LXR-Luc luciferase activity was investigated by dual luciferase reporter gene system,and the expression of LXRαprotein was detected by Western Blot.Results:Euphorbia factor L_(1),L_(2),L_(3),L_(7a),L_(7b)and L_(8)could significantly reduce the relative luciferase activity(RLU)of LXRα,and the expression level of LXRαprotein was significantly down-regulated.Conclusion:Euphorbia factor L_(1),L_(2),L_(3),L_(7a),L_(7b)and L_(8)can inhibit the expression of LXR protein level,which may be achieved by inhibiting the transcriptional activity of LXR.

关 键 词：Semen Euphorbiae diterpene esters HEK293 LXR dual luciferase reporter gene system 

分 类 号：R28[医药卫生—中药学]