抗磷脂抗体损害子宫蜕膜血管内皮细胞血管形成的机制研究  

Study on mechanism of antiphospholipid antibody damage to the angiogenesis capacity of decidual vascular endothelial cells

作　　者：张琳琳[1] 郑雪梅 王谢桐[2] 董艳[1] Zhang Linlin;Zheng Xuemei;Wang Xietong(Department of Obstetrics and Gynecology,Linyi People's Hospital,Linyi 276005;Department of Obstetrics and Gynecology,Shandong Provincial Hospital,Cheeloo College of Medicine,Shandong University,Jinan 250021)

机构地区：[1]临沂市人民医院妇产科,临沂276005 [2]山东大学附属省立医院妇产科,济南250021

出　　处：《现代妇产科进展》2024年第6期451-455,共5页Progress in Obstetrics and Gynecology

基　　金：国家自然科学基金面上项目(No:81873842);临沂市重点研发计划(医学类)(No:2022YX0092)。

摘　　要：目的:研究抗磷脂抗体(aPL)对蜕膜组织血管内皮细胞(DVEC)增殖、迁移与血管形成能力的影响。方法:采用细胞免疫荧光鉴定DVEC细胞;CCK-8法检测aPL对DVEC细胞增殖的影响;细胞划痕实验与血管形成实验检测aPL对DVEC细胞迁移与血管生成的影响;实时荧光定量PCR(RT-PCR)检测aPL对DVEC细胞基质金属蛋白酶2(MMP-2)和血管内皮生长因子(VEGF)mRNA表达的影响;Western blot检测aPL对DVEC细胞中p-ERK、t-ERK、VEGF与MMP-2蛋白表达的影响。结果:aPL能抑制DVEC细胞增殖、迁移与血管形成能力;aPL能抑制DVEC细胞中p-ERK、VEGF与MMP-2表达水平,加ERK激动剂后DVEC细胞中p-ERK、VEGF与MMP-2表达水平明显升高。结论:aPL可通过调控ERK通路减少DVEC细胞中VEGF与MMP-2表达,进而抑制DVEC的增殖、迁移与血管形成能力。Objective:To study the effect of antiphospholipid antibody(aPL)on the proliferation,migration and angiogenesis of decidual vascular endothelial cells(DVECs).Methods:DVECs were identified by immunofluorescence assay.CCK-8 assay was used to detect the effect of aPL on DVECs proliferation.The effect of aPL on DVECs migration and angiogenesis was observed by cell scratch assay and angiogenesis assay.Real-time PCR(RT-PCR)was used to detect the effect of aPL on MMP-2 and VEGF mRNA expression in DVECs.Western blot was used to detect the effect of aPL on ERK phosphorylation,VEGF and MMP-2 protein expression in DVECs.Results:aPL inhibited the proliferation,migration and angiogenesis of DVECs.aPL can inhibit ERK phosphorylation,VEGF and MMP-2 expression in DVECs.The expression levels of p-ERK,VEGF and MMP-2 in DVEC cells were significantly increased after the addition of ERK agonists.Conclusion:aPL can reduce the expressions of VEGF and MMP-2 in DVECs by regulating ERK pathway,and then inhibit the proliferation,migration and angiogenesis of DVECs.

关 键 词：抗磷脂抗体 蜕膜组织血管内皮细胞 ERK通路 血管形成 

分 类 号：R714[医药卫生—妇产科学]