阿里红黄酮减轻低氧力竭运动大鼠脑组织损伤的机制研究  

Study on the Mechanism of Brain Tissue Injury in Rats Induced by Hypoxic Exhaustive Exercise by Fomes Officinalis Ames Flavone

作　　者：孔海军 李新龙 KONG Haijun;LI Xinlong(College of Physical Education Science,Kashi Unversity,Kashi Xinjiang,844000)

机构地区：[1]喀什大学体育学院,新疆喀什844000

出　　处：《湖北体育科技》2024年第3期94-99,共6页Hubei Sports Science

基　　金：2020年新疆维吾尔自治区喀什地区科技计划(KS20200024)。

摘　　要：目的探讨阿里红黄酮(Fomes Officinalis Ames Flavone,FOF)对长期低氧力竭运动大鼠认知能力、神经炎症、小胶质细胞活化的影响。方法60只雄性大鼠,随机分为NC组、HE组、HE+LFOF组、HE+MFOF组和HE+HFOF组。HE组、HE+LFOF组、HE+MFOF组和HE+HFOF组大鼠接受连续6周力竭跑台运动,HE+LFOF组、HE+MFOF组和HE+HFOF组每次运动后30 min分别灌胃100、200和400 mg·kg^(-1)·d^(-1)阿里红黄酮。干预后评价大鼠自主活动和空间记忆能力;检测脑组织炎症、氧化应激反应及小胶质细胞活化情况。结果与HE组比较,HE+LFOF组、HE+MFOF组和HE+HFOF组运动总距离、跨越格栅数量、平均游泳速度、穿越平台次数和平台所在象限停留时间、脑组织SOD和GSH-px浓度均显著上升(p<0.05),中心区域活动时间、逃避潜伏期(1d~5d)、脑组织IL-1β、IL-6、TNF-α浓度和脑组织含水量、MDA、IL-1β、COX-2、Iba-1、GFAP蛋白表达均显著降低(p<0.05)。结论100~400 mg·kg^(-1)FOF可以抑制长期低氧力竭运动大鼠脑组织损伤并改善大鼠自主活动和空间记忆能力。Objective To investigate the effects of Fomes Officinalis Ames Flavone(FOF)on cognitive ability,neuroinflammation,and microglia activation in long-term hypoxic exhaustive exercise rats.Methods Sixty male rats were randomly divided into NC group,HE group,HE+LFOF group,HE+MFOF group and HE+HFOF group.The HE group,HE+LFOF group,HE+MFOF group,and HE+HFOF group rats received continuous 6 weeks of exhausted treadmill exercise.The HE+LFOF group,HE+MFOF group,and HE+HFOF group were gavaged with 100,200 and 400 mg·kg^(-1)·d^(-1)of Alirubin 30 minutes after each exercise.Evaluate the autonomous activity and spatial memory ability of rats after intervention.Detect brain tissue inflammation,oxidative stress response,and activation of microglia.Results Compared with HE group,the total exercise distance,number of grates crossed,average swimming speed,number of platform crossing,residence time in the quadrant where the platform was located,SOD and GSH-px concentrations in brain tissue were significantly increased in HE+LFOF group(p<0.05),HE+MFOF group and HE+HFOF group.The activity time of central region,the escape latency period(1d~5d),the concentration of IL-1β,IL-6 and TNF-αin brain tissue,the protein expressions of MDA,IL-1β,COX-2,Iba-1 and GFAP in brain tissue were significantly decreased(p<0.05).Conclusion FOF of 100~400 mg·kg^(-1)can inhibit brain tissue damage in long-term hypoxic exhaustive exercise rats and improve their autonomous activity and spatial memory abilities.

关 键 词：低氧力竭运动 阿里红黄酮 大鼠 脑组织损伤 

分 类 号：G804.7[文化科学—运动人体科学]