运动预干预对脑缺血再灌注大鼠学习记忆的影响及机制  

Effects of Exercise Preintervention on Learning and Memory and Synaptic Proteins Expression in Hippocampus CA1 of the Cerebral Ischemia Reperfusion Rats

作　　者：杨子欣 王艳红[2] 凌小妹 宋艳丽 苏晓云[3] 贺继平 YANG Zixin;WANG Yanhong;LING Xiaomei;SONG Yanli;SU Xiaoyun;HE Jiping(Department of Clinical Medicine,Heilongjiang Nursing College,Haerbin Heilongjiang,150086;Physical Education Institute,North University of China,Taiyuan Shanxi,030051;Fenyang College,Shanxi Medical University,Fenyang Shanxi,032299)

机构地区：[1]黑龙江护理高等专科学校临床医学系,黑龙江哈尔滨150086 [2]中北大学体育学院,山西太原030051 [3]山西医科大学汾阳学院护理系,山西汾阳032200

出　　处：《湖北体育科技》2024年第3期100-106,共7页Hubei Sports Science

基　　金：山西省自然科学基金项目(20210302123060)。

摘　　要：目的本研究主要通过测量4周跑台运动预干预对脑缺血再灌注(CI/R)大鼠脑梗死体积、海马组织炎症因子和海马CA1区突触素相关蛋白表达的影响,探讨跑台运动预干预改善CI/R大鼠认知功能的可能机制。方法40只SD雄性大鼠进行脑缺血再灌注(CI/R)造模,造模前CI/R运动组大鼠进行4周递增负荷跑台运动。CI/R 72 h后,评估所有大鼠学习记忆能力,测量大鼠脑梗死体积百分比、海马TNF-α、IL-6水平及海马CA1区SYN及PSD-95的表达。结果1)与假手术组比较,CI/R模型组大鼠水迷宫实验中逃避潜伏期显著增加,穿越平台次数显著下降;脑梗死体积%及海马组织中IL-6、TNF-α水平显著增加,海马CA1区SYN及PSD-95平均光密度值(MOD)显著下降。2)4周跑台运动预干预可显著改善CI/R大鼠学习记忆能力、脑梗死体积%及海马组织中TNF-α水平显著减少,海马CA1区SYN及PSD-95 MOD显著增加。结论跑台运动预干预可通过减轻海马神经炎症反应、减少脑梗死体积、增加突触相关蛋白表达,从而预防CI/R后的记忆缺陷,被认为是预防脑缺血性损伤的有效策略。Objetive The aim of the present study was to explore the possible mechanism of 4 weeks treadmill exercise preintervention in improving cognitive function of cerebral ischemia reperfusion(CI/R)rats by measuring the cerebral infarction volume,hippocampus inflammatory biomarkers levels and the synaptic proteins expression in hippocampus CA1.Methods 40 SD male rats were modeled for CI/R and the CI/R exercise group rats were subjected to 4 weeks of incremental load treadmill exercise before modeling.After 72 h of CI/R,the learning and memory ability of rats were evaluated,the cerebral infarction volume%,the level of TNF-α,IL-6 in hippocampus and the expression of SYN and PSD-95 in hippocampal CA1 region were measured.Results 1)Compared with the sham operation group,the escape latency significantly increased and the number of crossing the platform significantly decreased in the morris water mazet test;cerebral infarction volume%and the level of IL-6 and TNF-αin hippocampus significantly increased,while the MOD of SYN and PSD-95 in hippocampus CA1significantly decreased.2)The 4-week treadmill exercise preintervention can significantly improve the learning and memory ability of CI/R rats,the cerebral infarction volume%and the TNF-αlevel in hippocampus significantly decreased;the MOD of SYN and PSD-95 had a significant up-regulation in hippocampus CA1 region in CI/R exercise group rats when compared with the CI/R model group.Conclusion Treadmill exercise preintervention might prevent memory deficits after CI/R through reducing hippocampal neuroinflammatory response,reducing cerebral infarction volume and increasing the synaptic associated protein expression in hippocampal CA1,which is considered to be an effective strategy to prevent ischemic injury.

关 键 词：跑台运动预干预 脑缺血再灌注 学习记忆 海马炎症因子 突触素表达 

分 类 号：G804.7[文化科学—运动人体科学]