基于生物信息学探讨参附汤治疗慢性心力衰竭的机制  

Exploring the Action Mechanism of the Shenfu Decoction on Chronic Heart Failure Based on Bioinformatics

作　　者：左龙 高云水 李果 梁美丹 容伟明 朱松柏 杨毅 丁光远 刘杨 ZUO Long;GAO Yunshui;LI Guo;LIANG Meidan;RONG Weiming;ZHU Songbai;YANG Yi;DINGGuangyuan;LIU Yang(Yueyang Hospital of Traditional Chinese Medicine Affiliated of Hunan University of Chinese Medicine,Hunan Yueyang 414000,China;Guangxi University of Chinese Medicine,Guangxi Nanning 530000,China;Guangxi Zhuang Autonomous Region Brain Hospital,Guangxi Liuzhou 545007,China;Pingjiang Hospital of Traditional Chinese Medicine,Hunan Yueyang 414500,China)

机构地区：[1]湖南中医药大学附属岳阳市中医医院,湖南岳阳414000 [2]广西中医药大学,广西南宁530000 [3]广西壮族自治区脑科医院,广西柳州545007 [4]湖南省岳阳市平江县中医医院,湖南岳阳414500

出　　处：《中医药临床杂志》2024年第5期893-900,共8页Clinical Journal of Traditional Chinese Medicine

基　　金：湖南省中医药管理局项目(201983);湖南省科学技术厅(2020SK52805)。

摘　　要：目的:通过生物信息学方法挖掘参附汤治疗慢性心力衰竭(chronic heart failure,CHF)的有效成分、基因及信号通路,探讨其潜在作用机制。方法:利用TCMSP数据库检索参附汤中所含中药有效成分及作用靶点,通过GEO数据库筛选出慢性心力衰竭的芯片数据集并进行差异分析,获得CHF的相关基因再与中药作用的靶点做交集得到靶点基因;运用cytoscape3.8.2软件构建有效成分-靶点基因网络并筛选出重要的有效成分;最后构建靶点基因的蛋白互作网络并对靶点基因进行GO生物过程和KEGG通路富集分析。结果:从参附汤中有筛选出有效成分25个,与CHF相关的共同作用靶点基因是8个,重要的有效成分是山奈酚、人参皂苷rh2、β-谷甾醇;关键的靶点基因包括PTGS2、IL1B、JUN、NFKBIA、CASP1等;GO生物过程结果显示与机械刺激的反应、发热、对脂多糖的反应等相关;KEGG通路富集分析结果显示主要涉及C-型凝集素受体信号通路、IL-17信号通路及TNF信号通路。结论:该研究通过生物信息学分析参附汤治疗CHF的潜在作用机制,初步阐明了参附汤治疗CHF具有多成分、多靶点和多通路参与的特点,为后续深入研究提供理论基础。Objective:To explore the active ingredients,genes and signaling pathways of Shenfu Decoction in treating chronic heart failure(CHF)through bioinformatics methods and explore its potential mechanism.Methods:The TCMSP database was used to search for the active ingredients and targets of traditional Chinese medicine contained in Shenfu Decoction.The chip data set of chronic heart failure was screened out through the GEO database and differential analysis was performed.The related genes of CHF were obtained and then compared with the targets of traditional Chinese medicine.The target genes were obtained through intersection;the cytoscape3.8.2 software was used to construct the active ingredient-target gene network and screen out the important active ingredients;finally,a protein interaction network of the target genes was constructed and the target genes were enriched with GO biological processes and KEGG pathways.Set analysis.Results:25 active ingredients were screened out from Shenfu Decoction,and there were 8 common target genes related to CHF.The important active ingredients were kaempferol,ginsenoside rh2,andβ-sitosterol;the key target genes Including PTGS2,IL1B,JUN,NFKBIA,CASP1,etc.;GO biological process results show that it is related to the response to mechanical stimulation,fever,response to lipopolysaccharide,etc.;KEGG pathway enrichment analysis results show that it mainly involves C-type lectin receptor signaling pathway,IL-17 signaling pathway and TNF signaling pathway.Conclusion:This study analyzed the potential mechanism of action of Shenfu Decoction in treating CHF through bioinformatics,and initially clarified that Shenfu Decoction has the characteristics of multicomponent,multi-target and multi-pathway involvement in treating CHF,providing a theoretical basis for subsequent in-depth research.

关 键 词：生物信息学 参附汤 慢性心力衰竭 基因 信号通路 

分 类 号：R541[医药卫生—心血管疾病]