HPLC法测定金丹附延颗粒中肉桂酸和桂皮醛的含量  

Determination of Cinnamic Acid and Cinnamic Aldehyde in Jindan Fuyan Granules by HPLC

作　　者：朱艳艳 刘德鸿 吴倩颖 朱良辉 陈伟康 ZHU Yan-yan;LIU De-hong;WU Qian-ying;ZHU Liang-hui;CHEN Wei-kang(Jiangxi University of Traditional Chinese Medicine,Jiangxi Nanchang 330004,China;Jiangxi Institute for Drug Control,Jiangxi Engineering Research Center for Drug and Medical Device Quality,National Medical Products Administration Key Laboratory of Quality Evaluation of Traditional Chinese Patent Medicine,Jiangxi Nanchang 330029,China)

机构地区：[1]江西中医药大学,江西南昌330004 [2]江西省药品检验检测研究院,国家药品监督管理局中成药质量评价重点实验室,江西省药品医疗器械质量工程研究中心,江西南昌330029

出　　处：《中国药物评价》2024年第2期97-100,共4页Chinese Journal of Drug Evaluation

基　　金：江西省药品监督管理局2023年重点项目(编号:2023JS11)

摘　　要：目的:建立金丹附延颗粒中肉桂酸和桂皮醛的HPLC含量测定方法。方法:采用C 18色谱柱,流动相为乙腈和0.1%磷酸溶液,检测器检测波长设置为290 nm,输液泵流速1.0 mL·min^(-1),色谱柱柱温设置为25℃。结果:肉桂酸、桂皮醛分别在0.2079~10.3938μg·mL^(-1)、0.2025~10.1270μg·mL^(-1)范围内线性关系良好(r=1.0000),回收率分别为100.3%、102.6%,回收率RSD分别为1.57%、1.82%。结论:该方法简单、稳定,可用于金丹附延颗粒中肉桂酸和桂皮醛的含量测定。Objective:To establish a HPLC method for the determination of cinnamic acid and cinnamic aldehyde in Jindan Fuyan granules.Methods:Cinnamic acid and cinnamic aldehyde were separated by C 18 column with a mobile phase consisting of acetonitrile-0.1%phosphoric acid solution.The detection wavelength was set at 290 nm with a flow rate of 1.0 mL·min^(-1) and a chromatographic column temperature of 25℃.Results:Cinnamic acid and cinnamic aldehyde were present in the concentration range of 0.2079-10.3938μg·mL^(-1) and 0.2025-10.1270μg·mL^(-1)(r=1.0000),and the recoveries were 100.3%and 102.6%,RSDs were 1.57%and 1.82%,respectively.Conclusion:The method is simple,stable,and can be used for the determination of cinnamic acid and cinnamic aldehyde in Jindan Fuyan granules.

关 键 词：金丹附延颗粒 含量测定 肉桂酸 桂皮醛 高效液相色谱 

分 类 号：R284.1[医药卫生—中药学]