响应面优化提取新鲜沙棘果中异鼠李素及其两性聚合物结构修饰  

Response Surface Methodology Extraction of Isorhamnetin from Fresh Seabuckthorn and Design of Isorhamnetin Zwitterionic Polymers

作　　者：赵磊 丁雪[3] 孙艳杰 马金刚[4] 孟军[5] 姜大成 昝珂[6] 杨铭[7] ZHAO Lei;DING Xue;SUN Yan-jie;MA Jin-gang;MENG Jun;JIANG Da-cheng;ZAN Ke*;YANG Ming(Changchun University of traditional Chinese Medicine School of Medicine,Jilin Changchun130117,China;Siping Institute for Food and Drug Control,Jiling Siping 136000,China;Jilin Normal University College of Chemistry,Jilin Siping 136000,China;Siping Central People′s Hospital,Jiling Siping 136000,China;Jilin Medical Device Inspection and Research Institute,Jilin Changchun 130041,China;National Institutes for Food and Drug Control,Beijing 102629,China;Jining Reserarch Institute for Food and Drug Inspection and Testing,Shandong Jining 272000,China)

机构地区：[1]长春中医药大学药学院,吉林长春130117 [2]四平市食品药品检验所,吉林四平136000 [3]吉林师范大学化学学院,吉林四平136000 [4]四平市中心人民医院,吉林四平136000 [5]吉林省医疗器械检验研究院,吉林长春130041 [6]中国食品药品检定研究院,北京102629 [7]济宁市食品药品检验检测研究院,山东济宁272000

出　　处：《中国药物评价》2024年第2期141-147,共7页Chinese Journal of Drug Evaluation

基　　金：吉林省中药材标准,项目名称:新鲜沙棘果(JLYC-2021-024);吉林省发改委产业技术研究与开发项目(No.2020C037-3);吉林省道地药材实体标本库和数字化信息平台建设。

摘　　要：目的:建立响应面优化提取新鲜沙棘果中异鼠李素的方法,采用高分子两性聚合物修饰异鼠李素。方法:采用Design-Expert 8.0.6软件及其Box-Behnken优化从新鲜沙棘果中提取异鼠李素,制备的异鼠李素采用自组装模式制成两性聚合物。结果:优化的提取温度为75℃,提取时间60 min,100 g新鲜沙棘果中盐酸加入量35 mL;采用自组装模式制成异鼠李素两性聚合物反应完全,溶解度良好。结论:响应面法稳定可行,可用于新鲜沙棘果中异鼠李素工艺优化;自组装模式制成异鼠李素两性聚合物结构修饰可行。Objective:To establish a response surface optimization method for extracting isorhamnetin from fresh seabuckthorn fruits,and modify isorhamnetin with a high molecular amphoteric polymer.Methods:Design Expert 8.0.6 software and Box Behnken optimization were used to extract isorhamnetin from fresh seabuckthorn fruit,and the prepared isorhamnetin was self-assembled into an amphoteric polymer.Results:The optimized extraction temperature is 75℃,extraction time is 60 minutes,and the amount of hydrochloric acid added to 100g of fresh seabuckthorn fruit is 35 mL;The use of self-assembly mode to produce isothamnetin amphoteric polymer has complete reaction and good solubility.Conclusion:The response surface method is stable and feasible,and can be used to optimize the process of isorhamnetin in fresh seabuckthorn fruit;The self-assembly mode is feasible for the structural modification of isothamnetin amphoteric polymers.

关 键 词：响应面优化 新鲜沙棘果 异鼠李素 两性聚合物 

分 类 号：R931.6[医药卫生—生药学]