不同产地石吊兰药材UPLC指纹图谱及化学计量学分析  

Quality Analysis of Lysionotus pauciflorus from Different Origins by UPLC Coupled with Chemometrics

作　　者：刘趣 徐文芬 孙庆文 丁宁 王波 陆祥 Liu Qu;Xu Wenfen;Sun Qingwen;Ding Ning;Wang Bo;Lu Xiang(Guizhou University of Traditional Chinese Medicine,Guiyang 550025,China)

机构地区：[1]贵州中医药大学,贵州贵阳550025

出　　处：《广东化工》2024年第7期143-146,163,共5页Guangdong Chemical Industry

基　　金：贵州中医药大学一流学科建设研究生创新项目(GNYL[2017]008号-7-Y);贵州省中央引导地方科技发展资金项目(黔科中引地[2022]4016);贵州省优秀青年科技人才项目(黔科合平台人才(2019)5658)。

摘　　要：目的:建立不同产地石吊兰药材UPLC指纹图谱,结合相似度评价、聚类分析、主成分分析、正交偏最小二乘回归分析等化学计量手段评价不同产地石吊兰药材品质,为其质量控制研究提供方法参考和基础数据。方法:通过UPLC梯度洗脱建立65批次石吊兰药材的指纹图谱,采用中药色谱指纹图谱相似度评价系统(2012A版)对石吊兰UPLC指纹图谱进行相似度评价,并采用聚类分析、主成分分析以及正交偏最小二乘判别分析对石吊兰品质进行综合评价。结果:标定了15个共有峰,并指认了10个共有峰;65批样品与对照指纹图谱的相似度在0.316~0.954之间;聚类分析将65批样品分为两大类;主成分分析筛选出累积方差达79.202%贡献率的5个主成分,并通过各主成分载荷得到每个批次的综合得分,以反映综合质量;正交偏最小二乘判别分析筛选出黔产、滇产、桂产石吊兰的差异性成分为苯乙醇苷类和黄酮类成分。结论:建立的石吊兰UPLC指纹图谱结合化学计量学评价方法,简便可行,可为综合评价不同产地石吊兰药材的质量提供参考。Objective:To establish the fingerprints of Lysionotus pauciflorus from different habitats by UPLC,and comprehensively analyze the quality of L.pauciflorus by the similarity evaluation(SA)in combination with clustering,principal component analysis(PCA)and other chemo-metrics,so as to provide theoretical basis for its quality evaluation.Methods:The fingerprints of 65 batches of L.pauciflorus were established by UPLC gradient elution,and the similarity evaluation of the UPLC fingerprints of L.pauciflorus medicinal materials was carried out using the traditional Chinese medicine chromatographic fingerprint similarity evaluation system(2012A version).Different analysis,SA,PCA,and orthogonal partial least squares-discriminant analysis(OPLS-DA)were used to comprehensively evaluate the quality of L.pauciflorus.Results:Fifteen chromatographic peaks were selected as the common peaks,and 10 common peaks were identified.The similarity of the 65 batches was 0.316~0.954.PCA analysis screened out five principal components with cumulative variance contribution rate of 79.202%,and obtained the comprehensive score of each batch through the loading of each principal component,reflecting the comprehensive quality.The differential components of L.pauciflorus from Guizhou,Yunnan and Guizhou Provinces were screened out by OPLS-DA as phenylethanoid glycosides and flavonoids.Conclusion:UPLC fingerprint established in this experiment combined with the chemometric evaluation method is simple,feasible and reproducible,and can be used to comprehensively evaluate the quality of L.pauciflorus from different origins.

关 键 词：石吊兰 UPLC指纹图谱 化学计量学 连翘脂苷B 毛蕊花糖苷 石吊兰素 

分 类 号：R284[医药卫生—中药学]