稀释比例对绵羊精子活率、活力、运动速率、质膜完整率和顶体完整率的影响  

Effect of dilution ratio on total spermatozoa motility,progressive motility,kinematic parameters,plasma membrane integrity and acrosome integrity in sheep

作　　者：张柳明 王旭洋 江才雨 孙宇轩 王健[1] 孙晓梅 李拥军[1] ZHANG Liuming;WANG Xuyang;JIANG Caiyu;SUN Yuxuan;WANG Jian;SUN Xiaomei;LI Yongjun(College of Animal Science and Technology/Jiangsu Province Key Laboratory for Animal Genetics&Molecular Breeding,Yangzhou University,Yangzhou 225009,China)

机构地区：[1]扬州大学动物科学与技术学院/江苏省动物遗传繁育与分子设计重点实验室,江苏扬州225009

出　　处：《畜牧与兽医》2024年第6期15-21,共7页Animal Husbandry & Veterinary Medicine

基　　金：江苏现代农业产业技术体系建设项目(JAST[2023]448)。

摘　　要：旨在研究15℃条件下保存绵羊精液的最适宜稀释比例,获得较好的精液保存效果。试验分别将精液按照1:1、1:4、1:9、1:14、1:19和1:24进行稀释保存,在保存过程中检测精子的活率、活力、直线速率(VSL)、曲线速率(VCL)、路径速率(VAP)、侧摆幅度(ALH)、移动角度(MAD)、质膜完整率和顶体完整率。结果显示,保存3~6 d,1:9稀释后的精子活率显著高于其他稀释比例(P<0.05);保存1~5 d,1:4和1:9稀释后的精子活力显著高于其他稀释比例(P<0.05);保存3~5 d,1:4和1:9稀释后的精子质膜完整率和顶体完整率显著高于其他稀释比例(P<0.05);保存3~6 d,1:4和1:9稀释后的精子VCL、VAP和ALH显著高于其他稀释比例(P<0.05),但两组之间差异不显著(P>0.05)。因此,在本试验条件下,15℃保存绵羊精液最合适的稀释比例是1:9,过高和过低的稀释比例均不利于绵羊精液保存。This study was to determine the optimal dilution ratio for preserving sheep semen at 15℃and to achieve a better semen preser⁃vation effect.The semen samples were diluted at ratios of 1:1,1:4,1:9,1:14,1:19,and 1:24,respectively.Then,total sperma⁃tozoa motility,progressive motility,straight line velocity(VSL),curvilinear velocity(VCL),average path velocity(VAP),amplitude of lateral head displacement(ALH),average motion degree(MAD),plasma membrane integrity,and acrosome integrity of the samples were measured during preservation.The results showed that the total spermatozoa motility in the 1:9 dilution group was significantly(P<0.05)higher than that in the other dilution ratio groups,for 3-6 days of preservation.The progressive spermatozoa motility in the 1:4 and 1:9 di⁃lution groups was significantly higher than those in the other dilution ratio groups,for 1-5 days of preservation(P<0.05).During 3-5 days of preservation,the plasma membrane integrity and acrosome integrity were significantly higher in the 1:4 and 1:9 dilution groups,com⁃pared with the other dilution groups(P<0.05).During 3-6 days of preservation,VCL,VAP,and ALH in the 1:4 and 1:9 dilution groups were significantly higher than those in the other dilution ratio groups(P<0.05).However,the difference between the two groups was not significant(P>0.05).Therefore,based on the conditions of this experiment,the most appropriate dilution ratio for preserving sheep se⁃men at 15℃was 1:9.Both excessively high and low dilution ratios were found to be unfavorable for sheep semen preservation.

关 键 词：绵羊精液 稀释比例 15℃ 精子活力 

分 类 号：S826[农业科学—畜牧学]