用于H_(2)PO_(4)^(-)检测的比率型荧光探针合成及性能研究  

Synthesis and Properties of Ratiometric Fluorescent Sensor for H_(2)PO_(4)^(-)

作　　者：郭昌晟 陈君霞 刘忆茹 卢钰盛 董智云 席福贵 GUO Change-sheng;CHEN Jun-xia;LIU Yi-ru;LU Yu-sheng;DONG Zhi-yun;XI Fu-gui(Department of Chemistry,Xinzhou Teachers University,Xinzhou 034000,China)

机构地区：[1]忻州师范学院化学系,山西忻州034000

出　　处：《化学试剂》2024年第6期33-38,共6页Chemical Reagents

基　　金：山西省高等学校大学生创新创业训练计划项目(2022001);国家自然科学基金资助项目(21701137)。

摘　　要：设计合成了一种基于吖啶-苯并咪唑鎓的大环受体ABIM,可作为H_(2)PO_(4)^(-)的荧光比率传感器。借助荧光光谱研究了受体ABIM对H_(2)PO_(4)^(-)的选择性识别,在大环受体ABIM的乙腈溶液中加入H_(2)PO_(4)^(-)后,在426 nm处荧光发射明显“关闭”,506 nm处荧光发射明显“打开”,溶液荧光颜色由蓝色变为蓝绿色,新的荧光发射峰可能是由于H_(2)PO_(4)^(-)诱导两个受体中的吖啶环之间形成Excimer产生的。ABIM与H_(2)PO_(4)^(-)以1∶1的化学计量比结合,结合常数为(7.13±0.48)×10^(6)L/mol,检出限为2.38×10-7 mol/L,最佳响应时间为2 min。回收实验表明,ABIM可应用于样品中H_(2)PO_(4)^(-)的检测。An acridine-benzimidazolium based macrocyclic fluorescent sensor ABIM were designed and synthesized,which can be used as ratiometric fluorescent sensor for H_(2)PO_(4)^(-).The selectivity of sensor ABIM with H_(2)PO_(4)^(-)was investigated by fluorescence spectroscopy.A clear“turn-off”of fluorescence emission at 426 nm and an apparent“turn-on”of fluorescence emission at 506 nm were observed upon addition of H_(2)PO_(4)^(-),giving rise to change in the fluorescence color from blue to blue-green.The new fluorescence emission may be attributed to the H_(2)PO_(4)^(-)induced assembly of sensor ABIM forming the excimer conformation between two acridine rings.The results showed that the binding ratio for sensor ABIM to H_(2)PO_(4)^(-)was 1∶1.The binding constant was calculated to be(7.13±0.48)×10^(6)L/mol,the detection limit was 2.38×10-7 mol/L and the optimal response time was 2 minutes.The results also showed that sensor ABIM could be used to detection of H_(2)PO_(4)^(-)in the samples.

关 键 词：吖啶 苯并咪唑鎓 H 2PO-4 大环 比率型荧光探针 

分 类 号：O65[理学—分析化学]