氮掺杂的绿色碳点开关型荧光传感器灵敏检测组氨酸  

Detection of Histidine by Switch Sensitive Sensor Based on N-Doped Green Fluorescent CDs

作　　者：蔡留莹 康鹏 董微[2] CAI Liu-ying;KANG Peng;DONG Wei(School of Public Health,Shenyang Medical College,Shenyang 110034,China;Chemistry Teaching and Research Office,Shenyang Medical College,Shenyang 110034,China)

机构地区：[1]沈阳医学院公共卫生学院,辽宁沈阳110034 [2]沈阳医学院化学教研室,辽宁沈阳110034

出　　处：《化学试剂》2024年第6期46-52,共7页Chemical Reagents

摘　　要：以柠檬酸为碳源,尿素提供氮源,N,N-二甲基甲酰胺(DMF)为溶剂,采用水热法一步合成水溶性好的氮掺杂绿色荧光碳点(N-CDs)。为增强N-CDs的荧光强度,选用1,6-己二胺作为表面修饰剂,制备的N-CDs荧光量子产率达16.8%。该N-CDs形貌近似球形,平均粒径约为30 nm。同时本实验基于荧光猝灭法构建N-CDs/Bio“开-关”型荧光传感器,利用传感器荧光强度的变化灵敏检测组氨酸(His)。传感器荧光强度变化(ΔF)与His浓度在1.73~25.85 mmol/L范围内呈现良好线性关系,线性方程为ΔF=6.133×[His]+4.531,相关系数R 2=0.9984,检测限为0.3669 mmol/L。进一步检测实际人血清样本中的His,回收率为96.3%~100.1%。传感器检测方法简便、灵敏度高,是优异的His荧光检测传感器。We used citric acid as the carbon source,urea as the nitrogen source,and N,N-dimethylformamide(DMF)as the solvent to successfully prepare a green fluorescent CDs by the hydrothermal method.Simultaneously,1,6-hexanediamine was used as a modifier to significantly enhance the photoluminescence intensity of N-CDs and the fluorescence quantum yield can reach 16.8%.The N-CDs is approximate spherical morphology,and the average particles size of about 30 nm.At the same time,a N-CDs/Bio fluorescence sensor like“Switch”was constructed based on the fluorescence quenching method.The change in fluorescence intensity of sensor was used to detect histidine(His).The sensor has a good linear relationship in the range of 1.73~25.85 mmol/L.The linear equationΔF=6.133×[His]+4.531,the correlation coefficient R 2=0.9984,and the detection limit was 0.3669 mmol/L.Further detection of His in serum,the recovery rate was 96.3%~100.1%,the detection of sensor was simple and sensitive.This is an excellent His detection fluorescent probe.

关 键 词：绿色荧光碳点 组氨酸 水热法 开关 荧光传感器 

分 类 号：O657[理学—分析化学]