基于高分辨液质联用的双标记氨基酸同位素丰度测定方法研究  

Analysis of Isotope Abundance for Double-labelled Amino Acid with Liquid Chromatography-High Resolution Mass Spectrometer

作　　者：赵雅梦 徐增益 范若宁 解龙 侯静华 李良君 刘建军[3] 雷雯 ZHAO Ya-meng;XU Zeng-yi;FAN Ruo-ning;XIE Long;HOU Jing-hua;LI Liang-jun;LIU Jian-jun;LEI Wen(Shanghai Research Institution of Chemical Industry Co.Ltd.,Shanghai 200062,China;Shanghai Professional Technology Service Platform on Detection and Application Development for Stable Isotope,Shanghai 200062,China;Department of Nuclear Medicine,Renji Hospital,School of Medicine,Shanghai Jiao Tong University,Shanghai 200127,China)

机构地区：[1]上海化工研究院有限公司,上海200062 [2]上海市稳定同位素检测及应用研发专业技术服务平台,上海200062 [3]上海交通大学医学院附属仁济医院核医学科,上海200127

出　　处：《化学试剂》2024年第6期83-88,共6页Chemical Reagents

基　　金：国家重点研发计划项目(2021YFF0701900);上海市普陀区加快发展研发服务产业专项资金项目(202210-B01-05)。

摘　　要：近年来,稳定同位素标记化合物,尤其是同位素标记氨基酸在合成生物学、蛋白质组学、代谢组学等前沿科学研究中发挥着越来越重要的作用。建立了一种基于高分辨液质联用的双标记氨基酸同位素丰度检测方法,以^(13)C_(2),^(15)N-甘氨酸为例,考察了样品浓度、质谱扫描张数等影响因素对同位素丰度计算结果的影响,结果表明该方法具有良好的准确性和稳定性,所需样品用量少,操作简单,有望成为一种简便、通用的同位素试剂质控方法;也将为代谢组学、海洋学等同位素示踪技术应用研究提供数据支持。In recent years,stable isotope-labeled compounds,especially isotope-labeled amino acids are widely used in frontier scientific research such as synthetic biology,proteomics and metabolomics.An analytical method for the isotopic abundance calculation of^(13)C_(2),^(15)N double-labeled amino acids was established based on liquid chromatography-high resolution mass spectrometer(LC-HRMS).Application conditions,sample concentration and the number of scanned mass spectra were investigated.Results showed that the method had good accuracy and stability,required less sample and was easy to operate.It is expected to be a simple and generalized method for quality control of isotope reagents,and also to support frontier scientific research such as fermentation engineering and metabolomics.

关 键 词：双标记氨基酸 同位素丰度 高分辨液质联用仪 质量控制 13 C 2 15 N-甘氨酸 

分 类 号：O65[理学—分析化学]