药食两用植物黑老虎组培快繁体系研究  

Tissue Culture of Medicinal and Edible Plant Kadsura coccinea

作　　者：邓丽丽 蒋臻韬 莫燕兰 蒋向军 韦霄[1] 史艳财[1] DENG Lili;JIANG Zhentao;MO Yanlan;JIANG Xiangjun;WEI Xiao;SHI Yancai(Guangxi Institute of Botany,Chinese Academy of Sciences,Guilin 541006,China;Guilin Sunnylife Modern Bio-Tech INC.,Guilin 541000,China)

机构地区：[1]广西壮族自治区中国科学院广西植物研究所,广西桂林541006 [2]桂林亦元生现代生物技术有限公司,广西桂林541000

出　　处：《林业调查规划》2024年第3期152-156,共5页Forest Inventory and Planning

基　　金：国家林业和草原局重点研发项目(GLM [2021]037号);广西八桂青年拔尖人才项目;国家重点研发计划课题(No.2022YFF1300703)。

摘　　要：为改变药食同源植物黑老虎野生资源日趋减少、难以满足市场需求的现状,对组培快繁体系中的培养基配制及组培瓶苗培育条件进行了研究,以期建立黑老虎组培快繁体系,为规模化种苗繁殖奠定基础。结果表明,以MS+2.0 mg/L 6-BA+0.2 mg/L NAA为初代培养基,以B5+2.0 mg/L 6-BA+0.2 mg/L NAA为增殖培养基,以1/2 MS+0.1 mg/L 6-BA+1.5 mg/L NAA+0.3%活性炭为生根培养基,先暗培养,再光照培养,生根率可达100%,且小苗主根粗,长势强。组培苗最佳炼苗时间为15 d,最佳移栽基质为塘泥∶蔗渣∶鸡粪∶过磷酸钙=3.9∶4∶2∶0.1,最佳遮阳模式为半遮阳,最佳培育期为60 d。In view of the fact that the wild resources of Kadsura coccinea,a medicine and food homologous plant,are decreasing day by day,and it is difficult to meet the market demand,the preparation of culture medium and the cultivation conditions of tissue culture bottle seedlings in tissue culture rapid propagation system were studied,in order to establish a tissue culture rapid propagation system of K.coccinea and lay a foundation for large-scale seedling propagation.The results showed that MS+2.0 mg/L 6-BA+0.2 mg/L NAA was the primary culture medium,B5+2.0 mg/L 6-BA+0.2 mg/L NAA was the proliferation culture medium,and 1/2 MS+0.1 mg/L 6-BA+1.5 mg/L NAA+0.3%activated carbon was the rooting culture medium.The rooting rate could reach 100%,and the main root of the seedlings was thick and strong in growth when cultivated in dark and then under light.The best hardening time of tissue culture seedlings was 15 days,the best transplanting medium was pond mud∶bagasse∶chicken manure∶superphosphate=3.9∶4∶2∶0.1,the best shading mode was half shading,and the best cultivation period was 60 days.

关 键 词：黑老虎 无性繁殖 组培繁育 植物激素 生物基质 遮光处理 

分 类 号：S759.82[农业科学—森林经理学] S723.133[农业科学—林学]