黔北黑猪骨骼肌中肌纤维类型转化相关circRNAs的筛选  

Screening of circRNAs related to muscle fiber type conversion in skeletal muscle of Qianbei black pigs

作　　者：赵园园 孟金柱 宋兴超 安清明 吴震洋 ZHAO Yuanyuan;MENG Jinzhu;SONG Xingchao;AN Qingming;WU Zhenyang(College of Agricultural and Forestry Engineering and Planning,Tongren University,Tongren,Guizhou 554300,China;College of Veterinary Medicine,Hunan Agricultural University,Changsha,Hunan 410128,China)

机构地区：[1]铜仁学院农林工程与规划学院,贵州铜仁554300 [2]湖南农业大学动物医学院,湖南长沙410128

出　　处：《湖南农业大学学报（自然科学版）》2024年第2期71-77,共7页Journal of Hunan Agricultural University(Natural Sciences)

基　　金：贵州省教育厅青年科技人才成长项目(黔教合KY字[2020]166);贵州省科学技术厅高层次创新型人才培养项目(千层次)(2022-(2020)-044);贵州省科学技术厅重点实验室项目(黔科合平台人才[2020]2003号);铜仁市科技局科技支撑计划项目(铜市科研[2020]128号)。

摘　　要：选取9头6月龄健康黔北黑猪,随机分成3组,采集背最长肌(LDM)和腰大肌(PMM)肌肉组织,通过RNA-seq构建环状RNA(circRNAs)的表达谱,筛选差异表达的circRNAs,在黔北黑猪2种肌肉中共鉴定出63个差异表达circRNAs,其中31个在LDM中表达上调,32个表达下调;对差异表达circRNAs的源基因进行GO、KEGG富集分析,结果表明,差异表达的circRNAs的源基因主要富集在磷酸酶活性的调控、肌节组织活动、肌球蛋白-轻链-磷酸酶活性的正调控等条目;富集的KEGG通路中与肌纤维类型转化相关的通路包括肌动蛋白细胞骨架的调控、ECM-受体互作、PI3K-Akt信号通路。预测差异表达circRNA的靶miRNAs,并筛选对应的靶mRNAs,构建的circRNA-miRNA-mRNA调控网络显示,circ_0003749-miR-27a-MSTN、circ_0003749-miR-27a-PPARγ、circ_0012316-miR-23a-Myh1/2/4、circ_0007093-miR-214-3p-MEF2C、circ_0010118-miR-132-FoxO1和circ_0010118-miR-374a-5p-FoxO1可能通过ceRNA网络调控肌纤维类型的转化。Nine 6-month-old healthy Qianbei black pigs were randomly divided into 3 groups.The longissimus dorsi muscle(LDM)and psoas major muscle(PMM)were collected.The expression profiles of circRNAs were constructed by RNA-seq,and the differentially expressed circRNAs in LDM vs.PMM were screened.A total of 63 differentially expressed circRNAs were identified in the two muscles of Qianbei black pigs,of which 31 were up-regulated and 32 were down-regulated in LDM.GO enrichment and KEGG analysis were performed on the source genes of differentially expressed circRNAs.The results showed that the source genes of differentially expressed circRNAs were mainly concentrated in GO items such as the regulation of phosphatase activity,sarcomere activity,and positive regulation of myosin-light-chain-phosphatase activity.In the enriched KEGG pathway,the pathways involving muscle fiber type conversion include the regulation of actin cytoskeleton,ECM-receptor interaction,PI3K-Akt signaling pathway.Finally,target miRNAs of differentially expressed circRNAs were predicted,and corresponding target mRNAs of miRNAs were screened to construct the circRNA-miRNA-mRNA regulatory network.The results showed that circ_0003749-miR-27a-MSTN,circ_0003749-miR-27a-PPARγ,circ_0012316-miR-23a-Myh1/2/4,circ_0007093-miR-214-3p-MEF2C,circ_0010118-miR-132-FoxO1 and circ_0010118-miR-374a-5p-FoxO1 may regulate muscle fiber type conversion through ceRNA network.

关 键 词：黔北黑猪 骨骼肌 肌纤维类型转化 circRNA-miRNA-mRNA网络 

分 类 号：S828.1[农业科学—畜牧学]