空肠Ⅰ型闭锁隔膜组织黏膜层杯状细胞和黏蛋白2 表达的组织学观察  

Histological observation of goblet cell and mucoprotein 2 expression in mucosa of septumarisen from typeⅠintestinal atresia

作　　者：叶茂 何峰[2] 宋岩彪 高鹏 崔钊[5] 王欢[6] 刘雪来[7] Ye Mao;He Feng;Song Yanbiao;Gao Peng;Cui Zhao;Wang Huan;Liu Xuelai(Department of General Neonatal Surgery,Children's Hospital,Capital Institute of Pediatrics,Beijing 100020,China;Department of Biochemistry and Immunology,Capital Institute of Pediatrics,Beijing 100020,China;Department of Central Laboratory,the Second Hospital of Hebei Medical University,Hebei,Shijiazhuang050000,China;Department of Surgery,Heilongjiang Hospital,Beijing Children’s Hospital,CapitalMedical University,Heilongjiang,Harbin 150010,China;Department of Surgery,Changchun Children’sHospital,Jilin,Changchun 130061,China;Department of Pathology,Heilongjiang Hospital,BeijingChildren’s Hospital,Capital Medical University,Heilongjiang,Harbin 150010,China;Department ofGeneral Neonatal Surgery,Children's Hospital,Capital Institute of Pediatrics,Department of Physiology,Capital Institute of Pediatrics,Beijing 100020,China)

机构地区：[1]首都儿科研究所附属儿童医院普通新生儿外科,北京100020 [2]首都儿科研究所生化免疫学研究室,北京100020 [3]河北医科大学第二医院中心实验室,河北石家庄050000 [4]首都医科大学附属北京儿童医院黑龙江医院外科,黑龙江哈尔滨150010 [5]长春市儿童医院外科,吉林长春130061 [6]首都医科大学附属北京儿童医院黑龙江医院病理科,黑龙江哈尔滨150010 [7]首都儿科研究所附属儿童医院普通新生儿外科、首都儿科研究所生理学研究室,北京100020

出　　处：《发育医学电子杂志》2024年第3期199-203,共5页Journal of Developmental Medicine (Electronic Version)

基　　金：黑龙江省卫生健康委员会基金(2020-043);北京市自然科学基金(7222015);首都儿科研究所所级课题(LCYJ-2023-07)。

摘　　要：目的观察先天性空肠Ⅰ型闭锁隔膜组织黏膜层内的杯状细胞分布和黏蛋白2(mucoprotein 2,MUC2)的表达情况。方法选用距离Treitz韧带15 cm以内的空肠Ⅰ型闭锁患儿的隔膜组织(隔膜组),共收集组织标本9例。患儿手术年龄为出生后1~3 d。以同一患儿术中行肠切除肠吻合过程中钳取收集的正常肠壁组织为对照组。组织标本分别行苏木精-伊红(hematoxylin-eosin staining,HE)染色、过碘酸雪夫(periodic acid-Scheff staining,PAS)染色和免疫组织化学染色,并进行半定量比较。统计学方法采用独立样本t检验。结果HE染色显示,隔膜组织具备正常肠道的基本结构,绒毛数量减少、排列紊乱,黏膜肌层不规则,黏膜下层胶原纤维增生。隔膜组小肠绒毛间杯状细胞数量(36.6±2.6)个较对照组(72.5±3.7)个明显减少,差异有统计学意义(t=11.235,P=0.001)。PAS染色显示,杯状细胞内糖蛋白分泌增多。免疫组织化学染色显示,隔膜组黏膜层内MUC2光密度值(0.0850±0.0054)较对照组(0.1156±0.0144)减少,差异有统计学意义(t=18.652,P=0.014)。结论先天性空肠Ⅰ型闭锁隔膜与正常肠壁相比,其结构紊乱、绒毛减少,发育具有不完善性;隔膜组织由杯状细胞介导的黏膜防御功能较正常肠壁降低。Objective To observe the expression of goblet cell and mucoprotein 2(MUC2)in mucosa of septum arisen from typeⅠintestinal atresia.Method Totally 9 septa located in intestinal wall with distanceless than 15 cm from Treitz ligament(septum group)were collected.The age of the patients'operation were1-3 days after birth.The normal intestinal wall tissues were harvested and set as the control during resectionand anastomosis in the same patient(control group),followed by serial tissue sectioning and followinghematoxylin-eosin(HE)staining,periodic acid-Scheff(PAS)staining,as well immunohistochemistry stainingfor histological observation.The semi-quantitative calculation was conducted for statistical comparison.Statistical method performed by independent sample t test.Result HE staining showed mucous of septumarisen from typeⅠintestinal atresia presented relatively normal histological structure with less villi andmalalignment.Submucosa muscular layer was irregular and proliferating collagen in subcutaneous layer.Theresidual goblet cells were less in septum group(36.6±2.6)compared with control group(72.5±3.7),withthe difference was significant(t=11.235,P=0.001).PAS staining indicated that glycoprotein in goblet cell wasdenser and immunohistochemistry staining showed MUC2 was less-expressed in septum group(0.0850±0.0054)compared with control group(0.1156±0.0144),with the difference was significant(t=18.652,P=0.014).Conclusion Compared with the normal intestinal wall,the septum arisen from typeⅠintestinalatresia has disorganized microscopic structure and relatively less villi,and present imperfect developmentalfeature,suggesting weaker defensive function induced by goblet cell in contrast to the normal intestinal wall.

关 键 词：小肠 闭锁 隔膜 黏膜层 杯状细胞 黏蛋白2 

分 类 号：R726.5[医药卫生—儿科]