塔形栓皮栎体胚增殖与成熟的影响因素  

Embryo proliferation and maturation culture of Quercus variabilis Blume var.pyramidalis

作　　者：张婉 任跃 朱景乐[1,2] 刘学明 肖祥伟 柯书银 侯晓雅 ZHANG Wan;REN Yue;ZHU Jingle;LIU Xueming;XIAO Xiangwei;KE Shuyin;HOU Xiaoya(Henan Agricultural University,Zhengzhou 450003,Henan,China;Research Institute of Non-timber Forestry,Chinese Academy of Forestry,Key Laboratory of Economic Forest Germplasm Innovation and Utilization,State Forestry and grassland administration,Zhengzhou 450003,Henan,China;State-owned Tian Mu Mountain Forest Farm,Ping Qiao District,Xinyang 46400,Henan,China)

机构地区：[1]河南农业大学,河南郑州450003 [2]中国林业科学研究院经济林研究所种质创新与利用国家林业和草原局重点实验室,河南郑州450003 [3]国有信阳市平桥区天目山林场,河南信阳464000

出　　处：《中南林业科技大学学报》2024年第4期58-65,共8页Journal of Central South University of Forestry & Technology

基　　金：国家重点研发计划项目(2017YFD0600602-03);中央级公益性科研项目(CAFYBB2018ZB001-11)。

摘　　要：【目的】探究各因素在塔形栓皮栎体细胞胚的发育与成熟过程中的影响作用强弱,进一步完善塔形栓皮栎的体胚发生体系。【方法】以塔形栓皮栎合子胚诱导得到的胚性愈伤组织为材料,对影响塔形栓皮栎体胚增殖、成熟的主要因素进行探索:利用单因素试验筛选适合塔形栓皮栎体胚增殖的光照条件(强光、弱光、无光)、激素的种类(萘乙酸(NAA)、细胞分裂素6-苄基氨基嘌呤(6-BA)、生长素2,4-二氯苯氧乙酸(2,4-D);对影响体胚增殖的激素浓度及配比分别进行双因素试验分析;对影响体胚成熟的蔗糖浓度、活性炭浓度、ABA浓度进行单因素试验分析;最后对影响体胚成熟的激素浓度及配比进行双因素试验分析。【结果】1)三种不同光照强度中,强光环境下体胚增殖诱导率最高;2)不同植物激素配比对体胚增殖现象存在显著差异,0.10 mg/L 2,4-D与0.80 mg/L 6-BA增殖率最高为83.33%;3)蔗糖浓度为5.00%时,体胚的成熟率最高为77.80%;ABA浓度0.50 mg/L时,成熟率可达到最高为50.00%;活性炭浓度为1.00 g/L时,长势良好,成熟率达到57.10%;4)NAA与6-BA浓度同时为0.25 mg/L时,体胚的成熟率最高可达90.50%。【结论】1)塔形栓皮栎体胚在强光环境下增殖效果较好;2)最佳增殖培养基为:MS+(6.0 g/L)琼脂+(3.00%)蔗糖+2,4-D(0.10 mg/L)+6-BA(0.80 mg/L);3)最佳成熟培养基为:MS+(7.0 g/L)琼脂+(5.00%)蔗糖+NAA(0.25 mg/L)+6-BA(0.50 mg/L)+ABA(0.50 mg/L)+活性炭(1.50 g/L)。【Objective】In order to explore the influence of various factors on the development and maturation of Quercus variabilis Blume var.pyramidalis embryo,and further improve the embryogenesis system of Q.variabilis Blume var.pyramidalis.【Method】The main factors affecting the proliferation and maturation of Q.variabilis Blume var.pyramidalis embryo were explored by using the embryonic callus induced by Q.variabilis Blume var.pyramidalis embryo as materials:single-factor experiments were used to screen the types of light conditions(strong light,weak light,no light)and hormones(naphthaleneacetic acid(NAA),cytokinin 6-benzylaminopurine(6-BA),auxin 2,4-dichlorophenoxyacetic acid(2,4-D))suitable for the proliferation of Q.variabilis Blume var.pyramidalis embryo.The concentration and ratio of hormones affecting embryonic proliferation were analyzed by two-factor tests.Univariate experimental analysis was carried out on sucrose concentration,activated carbon concentration and ABA concentration affecting embryo maturation.Finally,a two-factor test was carried out on the concentration and ratio of hormones affecting embryonic proliferation.【Result】1)Among three different light intensities,the induction rate of embryonic proliferation was the highest in the strong light environment;2)There were significant differences in the proliferation of embryos by different plant hormone ratios,with the highest proliferation rate of 83.33%with 0.10 mg/L2,4-D and 0.80 mg/L 6-BA;3)When the sucrose concentration was 5.00%,the maturity rate of the embryo was the highest which was 77.80%;When the ABA concentration was 0.50 mg/L,the maturity rate reached up to 50.00%;When the concentration of activated carbon was 1.00g/L,the growth was good,and the maturity rate reached 57.10%;4)When the concentration of NAA and 6-BA was 0.25 mg/L at the same time,the maturity rate of embryo reached up to 90.50%.【Conclusion】1)The body embryo of Q.variabilis Blume var.pyramidalis has a good proliferation effect in a strong light environment;2)The o

关 键 词：塔形栓皮栎 增殖 成熟 激素浓度 蔗糖浓度 

分 类 号：S792.18[农业科学—林木遗传育种]