甲基法尼酯和蜕皮激素对凡纳滨对虾幼体存活、变态以及Ⅱ型CHH基因表达的影响  

EFFECTS OF MF AND 20E ON LARVAL SURVIVAL,METAMORPHOSIS,AND EXPRESSION OF TYPE-II CHH GENES IN LITOPENAEUS VANNAMEI

作　　者：孙金凤 邓之通 刘飞 陈锚 杨颖飞 王忠凯 李玉全[1] SUN Jin-Feng;DENG Zhi-Tong;LIU Fei;CHEN Mao;YANG Ying-Fei;WANG Zhong-Kai;LI Yu-Quan(School of Marine Science and Engineering,Qingdao Agricultural University,Qingdao 266109,China;Bohai Seafoods Co.,Ltd.,Binzhou 251907,China;Shandong Jingming Ocean Science and Technology Co.,Ltd.,Dongying 257000,China)

机构地区：[1]青岛农业大学海洋科学与工程学院,山东青岛266109 [2]渤海水产股份有限公司,山东滨州251907 [3]山东景明海洋科技股份有限公司,山东东营257000

出　　处：《海洋与湖沼》2024年第3期746-755,共10页Oceanologia Et Limnologia Sinica

基　　金：国家自然科学基金项目,32102762号;山东省现代农业产业技术体系虾蟹类创新团队,SDAIT-15-011号;山东省科技型中小企业创新能力提升工程项目,2022TSGC2592号;东营市科技创新重大专项,2023ZDJH30号。

摘　　要：甲基法尼酯(MF)和蜕皮激素(20E)在甲壳动物的蜕皮、幼虫变态、形态发生过程中发挥重要作用。MF和20E的体内合成和分泌受到II型甲壳动物高血糖激素(CHH)的调控。为探讨MF和20E对凡纳滨对虾(Litopenaeus vannamei)幼体发育的影响,使用不同浓度的MF和20E处理不同发育时期的凡纳滨对虾幼体,并检测了幼体的存活率和变态率变化;鉴定了凡纳滨对虾II型CHH亚家族的全部成员,并使用实时荧光定量PCR技术检测了在外源MF和20E作用下,II型CHH基因在凡纳滨对虾仔虾的表达变化。研究发现,凡纳滨对虾的幼体早期阶段对MF和20E都表现出很强的敏感性,其存活率均呈剂量依赖性下降,并且MF和20E也能造成幼体变态率的降低。凡纳滨对虾II型CHH亚家族包含8个蜕皮抑制激素MIH基因(LvMIH1-8)和1个卵黄发生抑制激素VIH基因(LvVIH),在仔虾中LvMIH3表达量最高,其次是LvMIH1和LvMIH7,其余基因有微弱表达或者不表达。在外源MF和20E作用下,LvMIH1和LvMIH7的表达趋势类似,二者同时具有MIH和MOIH的功能特征,但LvMIH1侧重于抑制蜕皮,LvMIH7侧重于抑制MF合成;而LvMIH3参与蜕皮调控,不参与调控MF的合成。研究结果表明LvMIH1、LvMIH3、LvMIH7在凡纳滨对虾应答MF和20E信号中发挥重要作用,该结果对于深入认识II型CHH在甲壳动物蜕皮和变态发育中的调控机制具有重要意义。Methyl farnesoate(MF)and 20-hydroxyecdysone(20E)play crucial roles in the molting,metamorphosis,and developmental morphogenesis of crustaceans.The synthesis and secretion of MF and 20E in vivo are regulated by type-II crustacean hyperglycemic hormone(CHH).To investigate the impact of MF and 20E on the development of Litopenaeus vannamei larvae,different concentrations of MF and 20E were used to treat L.vannamei larvae of various developmental stages,and the survival rate and metamorphosis rate of the larvae were examined.Additionally,we conducted a comprehensive characterization of all members of the type-II CHH subfamily and assessed their expression patterns in post-larval shrimp in response to exogenous MF and 20E using real-time quantitative PCR.Results indicate that the early-stage larvae exhibited a heightened sensitivity to both MF and 20E.The survival rates decreased in a dose-dependent manner,and both MF and 20E also led to a reduction in metamorphosis rates.The type-II CHH subfamily of L.vannamei consists of eight molt-inhibiting hormone(MIH)genes(LvMIH1-8)and one vitellogenesis-inhibiting hormone(VIH)gene(LvVIH).In post-larval shrimp,the expression level of LvMIH3 was found the highest,followed by LvMIH1 and LvMIH7,while no significant expression of other type-II CHH genes was observed.Additionally,the application of exogenous MF and 20E significantly upregulated the expression of LvMIH1 and LvMIH7,whereas downregulated that of LvMIH3.Our findings suggest that LvMIH1,LvMIH3,and LvMIH7 play crucial roles in mediating the signaling of MF and 20E,providing valuable insights into the regulatory mechanism of type-II CHH in crustacean molting and metamorphosis.

关 键 词：凡纳滨对虾 甲基法尼酯 蜕皮激素 CHH 

分 类 号：Q789[生物学—分子生物学] S968[农业科学—水产养殖]