圈养高山兀鹫肠道微生物分离鉴定与抗生素耐药性分析  

Isolation,Identification and Antibiotic Resistance Analysis of Gut Microbiota from Captive Gyps himalayensis

作　　者：李凤 冯文启 郑思思 何顺福 赵文信 王稳 LI Feng;FENG Wenqi;ZHENG Sisi;HE Shunfu;ZHAO Wenxin;WANG Wen(State Key Laboratory of Plateau Ecology and Agriculture,Qinghai University,Xining Qinghai 810016;Animal Disease Prevention and Control Center of Qinghai Province,Xining Qinghai 810000;Xining Wild Animal Epidemic Disease Monitoring Station,Xining Qinghai 810001)

机构地区：[1]青海大学省部共建三江源生态与高原农牧业国家重点实验室,青海西宁810016 [2]青海省动物疫病预防控制中心,青海西宁810000 [3]西宁市野生动物疫源疫病监测站,青海西宁810001

出　　处：《现代农业科技》2024年第11期169-174,共6页Modern Agricultural Science and Technology

基　　金：国家自然科学基金地区科学基金项目:(31960277);青海大学生态环境工程学院一流本科专业科技创新项目(2022-stxy-B52)。

摘　　要：高山兀鹫是国家Ⅱ级保护鸟类,被世界自然保护联盟列入濒危物种红色名录。本文对圈养高山兀鹫肠道微生物进行分离与培养、16S rRNA基因扩增与测序、抗生素耐药性分析试验。结果表明:经鉴定,分离出的菌株隶属于2门、8属、9种,分别是弗氏柠檬酸杆菌(Citrobacter freundii)、太平洋芽孢杆菌(Bacillus pacificus)、粪肠球菌(Enterococcus faecalis)、蜂房哈夫尼亚菌(Hafnia alvei)、豪氏变形杆菌(Proteus hauseri)、普通变形杆菌(Proteus vulgaris)、腐败希瓦氏菌(Shewanella putrefaciens)、运动肉杆菌(Carnobacterium mobile)、福氏志贺氏菌(Shigella flexneri),这9种菌株对部分抗生素表现出耐药性,其中,运动肉杆菌、粪肠球菌和普通变形杆菌可耐受大多数抗生素,福氏志贺氏菌可耐受的抗生素种类较少。本文研究结果可为高山兀鹫的保护性人工养殖提供参考。Gyps himalayensis is a national ClassⅡprotected birds in China and is listed in the Red List of Threatened Species by the International Union for Conservation of Nature.This paper conducted isolation and cultivation,16S rRNA gene amplification and sequencing,and antibiotic resistance analysis experiments on gut microbiota of captive Gyps himalayensis.The results showed that the isolated strains belonged to 2 phyla,8 genera and 9 species,namely Citrobacter freundii,Bacillus Pacificus,Enterococcus faecalis,Hafnia alvei,Proteus hauseri,Proteus vulgaris,Shewanella putrefaciens,Carnobacterium mobile and Shigella flexneri.The nine strains showed resistance to a range of some antibiotics.Among them,Carnobacterium mobile,Enterococcus faecalis and Proteus vulgaris could tolerate most antibiotics.Shigella flexneri could tolerate fewer types of antibiotics.The research results of this paper can provide references for the protective artificial breeding of Gyps himalayensis.

关 键 词：圈养高山兀鹫 肠道微生物 16S rRNA基因鉴定 抗生素耐药性 

分 类 号：S852.61[农业科学—基础兽医学]