玉米ZmEREB46基因调控耐渍性功能探究  

Function of ZmEREB46 gene in maize regulating waterlogging tolerance

作　　者：唐开媛 梁昆 张彦军[2] 王兴荣[2] 邱法展[1] TANG Kaiyuan;LIANG Kun;ZHANG Yanjun;WANG Xingrong;QIU Fazhan(National Key Laboratory of Crop Genetic Improvement/Hubei Hongshan Laboratory,Huazhong Agricultural University,Wuhan 430070,China;Institute of Crops,Gansu Academy of Agricultural Sciences,Lanzhou 730070,China)

机构地区：[1]华中农业大学作物遗传改良全国重点实验室/湖北洪山实验室,武汉430070 [2]甘肃省农业科学院作物研究所,兰州730070

出　　处：《华中农业大学学报》2024年第3期139-147,共9页Journal of Huazhong Agricultural University

基　　金：国家自然科学基金项目(31960405);武汉市生物育种关键技术及新品种培育重大专项(2022021302024852);甘肃省农业科学院科研条件建设及成果转化项目(2021GAAS04);甘肃省省级重点人才项目(农作物种质资源保护利用与人才培养)。

摘　　要：为鉴定和发掘玉米渍水胁迫抗性基因,克隆玉米ERF家族成员ZmEREB46(Zm00001d015759)基因,同时对该基因进行重测序分析、功能变异位点鉴定以及表达模式分析,并进一步在模式植物拟南芥中初步探究ZmEREB46参与耐渍的功能。结果显示,ZmEREB46编码1个AP2/EREBP类转录因子;相较于耐渍自交系A3237,ZmEREB46基因编码区及启动子区在渍水敏感自交系A3239中分别存在1个G/A的转换以及1个911 bp片段插入,911bp片段的插入显著抑制了渍水敏感自交系A3239中ZmEREB46基因的表达;亚细胞定位结果显示,ZmEREB46定位在细胞核中;荧光定量PCR结果显示,ZmEREB46受渍水胁迫诱导上调表达,渍水处理8 h后ZmEREB46在耐渍自交系A3237中的表达量是渍水敏感自交系A3239中的2倍。结果表明,ZmEREB46在拟南芥中过量表达提高了拟南芥苗期的耐渍性。The ZmEREB46(Zm00001d015759)gene as a member of the ERF family in maize was cloned to identify and mine the resistance genes to waterlogging stress in maize.The resequencing,functional mutation site and expression pattern of the ZmEREB46 gene were analyzed.The role of the ZmEREB46 gene in waterlogging tolerance in Arabidopsis thaliana was preliminarily studied.The results showed that ZmEREB46 encoded one AP2/EREBP transcription factor.There was a G/A conversion and a 911 bp fragment insertion in the coding and promoter regions of the ZmEREB46 gene in the waterlogging susceptible inbred line A3239,compared to the waterlogging tolerant inbred line A3237.The insertion of a 911 bp fragment in the waterlogging susceptible inbred line A3239 significantly inhibited the expression of ZmEREB46.The results of subcellular localization showed that ZmEREB46 was localized in the nucleus.The results of quantitative PCR(qRT-PCR)analysis showed that ZmEREB46 was up-regulated by waterlogging stress,and the expression level of ZmEREB46 in A3237 was two-fold higher than that in A3239 after 8 h of waterlogging treatment.The overexpression of ZmEREB46 in A.thaliana improved the waterlogging tolerance of A.thaliana at the stage of seedling.

关 键 词：玉米 渍水胁迫 乙烯响应因子 ZmEREB46 转基因拟南芥 

分 类 号：S513.103.4[农业科学—作物学]