枯草芽孢杆菌复合微生物制剂对鸡坏死性肠炎的预防效果  

Preventive effect of Bacillus subtilis compound microbial preparation on chicken necrotic enteritis

作　　者：楚玉婷 朱阳华 谢长清[1] 程国富[1] 胡薛英[1] 张万坡[1] 谷长勤[1] CHU Yuting;ZHU Yanghua;XIE Changqing;CHENG Guofu;HU Xueying;ZHANG Wanpo;GU Changqin(College of Veterinary Medicine,Huazhong Agricultural University,Wuhan 430070,China)

机构地区：[1]华中农业大学动物医学院,武汉430070

出　　处：《华中农业大学学报》2024年第3期275-281,共7页Journal of Huazhong Agricultural University

基　　金：国家肉鸡产业技术体系项目(CARS-41);湖北省农业科技创新中心项目(2019-620-000-001-17)。

摘　　要：为了研发有效预防鸡坏死性肠炎(necrotic enteritis,NE)的微生态制剂,选用1日龄817雏鸡120只,随机分为4个处理组,其中,空白对照(Control)组和坏死性肠炎(NE)组(用G型产气荚膜梭菌感染肉仔鸡建立NE模型)饲喂基础日粮、预防1(Prob1)组在基础日粮中添加0.2%的微生物制剂1、预防2(Prob2)组在基础日粮中添加0.2%的微生物制剂2;试验第14~16天Prob1组、Prob2组和NE组肉鸡连续灌胃产气荚膜梭菌(Clos-tridium perfringens,CP),探究在饲料中添加不同的枯草芽孢杆菌复合微生物制剂对该病的预防作用。结果显示:NE组肉鸡体质量低于Control组,试验第17、23天Prob1组、Prob2组体质量显著高于NE组(P<0.05)。NE组肉鸡空肠上皮细胞坏死脱落、绒毛变短、隐窝加深、绒隐比降低,使用枯草芽孢杆菌复合微生物制剂后肉鸡空肠绒毛结构较完整、绒毛长度和绒隐比显著高于NE组。NE组肉鸡空肠黏膜T-SOD、T-AOC、AKP活性低于Control组;试验第17天Prob1组、Prob2组肉鸡空肠黏膜T-AOC、AKP活性显著高于NE组,Prob1组肉鸡空肠黏膜T-SOD活性显著高于NE组;试验第23天,Prob1组、Prob2组肉鸡空肠黏膜T-SOD、T-AOC、AKP活性高于NE组,无显著差异;NE组肉鸡空肠黏膜MDA含量高于Control组,试验第17、23天Prob1组、Prob2组肉鸡空肠黏膜MDA含量低于NE组。NE组肉鸡的肠道紧密连接蛋白CLDN1、ZO-2基因表达量低于Control组,在试验第17天有显著差异;试验第17、23天,Prob1组、Prob2组肉鸡肠道紧密连接蛋白CLDN1、ZO-2基因表达量高于NE组,试验第23天Prob1组、Prob2组ZO-1基因表达量显著高于NE组。研究表明,日粮中添加枯草芽孢杆菌复合微生物制剂可以改善坏死性肠炎鸡的生长发育、提高肉鸡抗氧化能力以及肠道紧密连接蛋白基因表达量,且枯草芽孢杆菌复合微生物制剂1对鸡坏死性肠炎有较好的预防作用。In order to find effective microecological preparations for preventing necrotic enteritis(NE)in chickens in this study,a total of 120 one-day-old 817 chickens were randomly divided into four treatment groups.Control group and NE group(broiler model of NE established by infecting with Clostrid-ium perfringens type G)were fed a basal diet.Probiotic group(Prob1)was fed a basal diet supplemented with 0.2%microbial agent 1,and probiotic group(Prob2)was fed a basal diet supplemented with 0.2%microbial agent 2.On days 14 to 16,broilers in Prob1,Prob2 and NE groups were treated with Clostridi-um perfringens(CP)by continuous gavage.The preventive effects of the two different Bacillus subtilis compound microbial preparations on NE of broilers were evaluated in the 23-day trial period.The results showed that,on day 17,the body weight of broilers in the NE group was lower than that of the control group.However,broilers in the Prob1 and Prob2 groups had a higher body weight at the 17th and 23rd days of the experiment compared to the NE group.The jejunal epithelial cells of broilers in the NE group were necrotic and exfoliated,with a shorter villus,deeper crypt,and smaller villus length and villus crypt ratio compared to the NE group treated with B.subtilis compound microbial preparation.The activities of T-SOD,T-AOC and AKP in the jejunal mucosa of broilers in the NE group were lower than those in the Control group.However,on day 17,the T-AOC and AKP activities in the jejunal mucosa of broilers in the Prob1 and Prob2 groups were significantly higher than those in the NE group,and the T-SOD activity of jejunal mucosa of broilers in Prob1 group was significantly higher than that in NE group.On day 23,the activities of T-SOD,T-AOC and AKP in jejunal mucosa of broilers in the Prob1 and Prob2 groups were higher than those in the NE group,and there were no significant differences.The content of MDA in jejunal mucosa of broilers in the NE group was higher than that in the control group,and the content of MDA in the Prob1 and Prob2 g

关 键 词：鸡 枯草芽孢杆菌复合微生物制剂 坏死性肠炎 产气荚膜梭菌 

分 类 号：S852.3[农业科学—基础兽医学]