小粒径Fe_(3)O_(4)-DMSA-PEI磁性纳米颗粒的制备及其基因负载能力研究  

Fe_(3)O_(4)-DMSA-PEI Magnetic Nanoparticles with Small Particle Size:Preparation and Gene Loading

作　　者：蔡和庆 韩璐[1] 杨松松 薛新玉 张扣 孙志成[1] 刘儒平 胡堃[1] 危岩[2] CAI Heqing;HAN Lu;YANG Songsong;XUE Xinyu;ZHANG Kou;SUN Zhicheng;LIU Ruping;HU Kun;WEI Yan(School of Printing and Packaging Engineering,Beijing Institute of Graphic Communication,Beijing 102600,China;Key Laboratory of Organophosphorus Chemistry and Chemical Biology,Tsinghua University,Beijing 100084,China)

机构地区：[1]北京印刷学院印刷与包装工程学院,北京102600 [2]清华大学生命有机磷化学及化学生物学教育部重点实验室,北京100084

出　　处：《无机材料学报》2024年第5期517-524,共8页Journal of Inorganic Materials

基　　金：国家自然科学基金(61971049,62211530446,22278037);北京印刷学院科研创新团队项目(Ea202205);北京市高等教育学会2023年立项重点课题(ZD202312)。

摘　　要：四氧化三铁(Fe_(3)O_(4))磁性纳米颗粒因其制备简单,在外加磁场作用下具有靶向性,并且表面易接枝等特性,可作为被动靶向载体应用于基因治疗领域。本研究采用溶剂热法制备纳米颗粒,并调控堆积生长时间,制得粒径在4~9 nm范围内可控的油相Fe_(3)O_(4)纳米颗粒;使用内消旋-2,3-二巯基丁二酸(DMSA)二次取代其表面的油酸分子,使其具备良好的水相分散性;通过酰胺化反应在其表面接枝支链型聚乙烯亚胺(PEI),最终得到Fe_(3)O_(4)-DMSA-PEI磁性纳米颗粒。研究发现,Fe_(3)O_(4)-DMSA-PEI磁性纳米颗粒的表面Zeta电位高达(52.50±1.94)mV,具有一定的超顺磁性(14.48 emu/g,1 emu/g=1 A·m^(2)/kg)。磁性纳米颗粒与质粒DNA的质量比为15:1时可完全阻滞DNA在凝胶上的电泳,装载量高达6.67%。本研究制备的Fe_(3)O_(4)-DMSA-PEI磁性纳米颗粒具有一定的基因负载能力,有望作为基因载体应用于基因转染领域。Ferroferric oxide(Fe_(3)O_(4))magnetic nanoparticles are widely used as passive targeting carriers in gene therapy,due to their simple preparation,targeting under external magnetic field and easy surface grafting.This study synthesized oil phase Fe_(3)O_(4)nanoparticles with controllable particle sizes in the range from 4 to 9 nm by regulating the accumulation growth time in the solvothermal method.Then,meso-2,3-dimercaptosuccinic(DMSA)was employed to double exchange oleic acid molecules on its surface to provide good water dispersibility.Finally,Fe_(3)O_(4)-DMSA-PEI magnetic nanoparticles were obtained by grafting branched polyethylenimine(PEI)onto Fe_(3)O_(4)-DMSA surface through amidization reaction.The results demonstrate that the Fe_(3)O_(4)-DMSA-PEI magnetic nanoparticles have a surface Zeta potential of(52.50±1.94)mV,remaining a certain degree of superparamagnetism(14.48 emu/g,1 emu/g=1 A·m^(2)/kg).When the mass ratio of Fe_(3)O_(4)-DMSA-PEI magnetic nanoparticles to plasmid DNA is 15:1,it can completely block DNA and its loading capacity is as high as 6.67%.The Fe_(3)O_(4)-DMSA-PEI magnetic nanoparticles prepared in this study have a certain gene delivery ability and are expected to be used as gene carriers in the field of gene transfection.

关 键 词：四氧化三铁 磁性纳米颗粒 基因载体 表面改性 

分 类 号：TB383[一般工业技术—材料科学与工程]