榼藤子对组胺急性痒小鼠的治疗作用研究  

The therapeutic effect of〝Ke-Teng-Zi〞on histamine-induced mice with acute itching

作　　者：鞠炎锟 和金凤 闫婷 熊慧[1] 梅之南[1] JU Yankun;HE Jinfeng;YAN Ting;XIONG Hui;MEI Zhinan(School of Pharmacy,South-Central Minzu University,Wuhan 430074,China)

机构地区：[1]中南民族大学药学院,武汉430074

出　　处：《中南民族大学学报（自然科学版）》2024年第4期461-467,共7页Journal of South-Central University for Nationalities：Natural Science Edition

基　　金：国家重点研发计划(2022YFC3502202);湖北省自然科学基金资助项目(2023AFB755)。

摘　　要：为了研究榼藤子提取物对组胺急性痒小鼠的治疗作用,通过组胺诱导小鼠急性痒模型,并将造模成功的小鼠随机分为模型组,榼藤子提取物低(25.0 mg/kg)、中(50.0 mg/kg)、高(100.0 mg/kg)剂量组,阳性药苯海拉明组(10.0 mg/kg).结果表明:榼藤子提取物可以降低组胺急性痒小鼠挠痒次数;明显改善皮肤表皮增厚;减少炎性细胞浸润;减少肥大细胞数量.此外,榼藤子提取物可以下调皮肤中H1R、TRPV1、IL-4、IL-6、IL-1β、IL-33、TNF-α、TSLP以及DRG中痒相关因子受体的基因表达水平,降低皮肤中H1R,TSLP与IL-4的蛋白含量.榼藤子提取物可以明显缓解组胺急性痒小鼠的症状,可能与其下调痒相关因子的基因与蛋白表达水平有关.To study the therapeutic effect of“Ke-Teng-Zi”(The seeds of Entada phaseoloides)extract on histamine-induced mice with acute itching,the successful histamine-induced acute itch mice were randomly divided into model group,“Ke-Teng-Zi”groups at 25.0,50.0,and 100.0 mg/kg dosage,and positive control group(diphenhydramine as positive drug,10.0 mg/kg).The results showed that the extract of“Ke-Teng-Zi”could reduce the number of scratches in histamine-induced mice with acute itching,significantly improve skin epidermal thickening,and relieve the infiltration of inflammatory cells and declined the number of mast cells.Furthermore,the extract of“Ke-Teng-Zi”could down-regulate the gene expression of HIR,TRPV1,IL-4,IL-6,IL-1β,IL-33,TNF-α,TSLP in skin and itch-related factor receptors in DRG,as well as decreasing the content of H1R,TSLP and IL-4 in the skin tissue.The extract of“Ke-Teng-Zi”could significantly alleviate the symptoms of mice with acute itching induced by histamine,and the mechanism may relate to the down-regulation of gene and protein expression of itching factors.

关 键 词：榼藤子 组胺 急性痒 炎性因子 

分 类 号：R285.5[医药卫生—中药学]