补骨脂异黄酮通过miR-497-5p调控对瘢痕疙瘩成纤维细胞增殖和迁移的机制研究  

Mechanism Study on Proliferation and Migration of Keloid Fibroblasts Regulated by Psoralen Isofl avones Via miR-497-5p

作　　者：梁博[1] 李国东 牟江月 崔昭[1] LIANG Bo;LI Guodong;MOU Jiangyue;CUI Zhao(Department of Dermatology,Baoding Second Hospital,Baoding 071051,Hebei,China;Department of Gastroenterology,Baoding First Central Hospital,Baoding 071030,Hebei,China)

机构地区：[1]保定市第二医院皮肤科,河北保定071051 [2]保定市第一中心医院消化三科,河北保定071030

出　　处：《中国美容医学》2024年第6期60-64,共5页Chinese Journal of Aesthetic Medicine

摘　　要：目的:探讨补骨脂异黄酮通过调控miR-497-5p抑制瘢痕疙瘩成纤维细胞增殖和迁移的机制研究。方法:体外培养瘢痕疙瘩成纤维细胞,使用浓度为0、9、18、36μg/ml补骨脂异黄酮处理细胞,记为Con组、低剂量组、中剂量组、高剂量组;按照脂质体法miR-NC、miR-497-5p转染细胞,记为miR-NC组、miR-497-5p组;按照脂质体法anti-miR-NC、anti-miR-497-5p转染细胞后,使用36μg/ml补骨脂异黄酮处理细胞,记为高剂量组+anti-miR-NC组、高剂量组+anti-miR-497-5p组。CCK8、克隆实验检测细胞增殖;伤口愈合实验检测细胞迁移;Western blot实验检测Cyclin D1、MMP2、MMP9蛋白表达水平;qRT-PCR实验检测miR-497-5p表达水平。结果:补骨脂异黄酮呈剂量关系降低瘢痕疙瘩成纤维细胞活性、迁移率(P<0.05),减少克隆细胞数(P<0.05),降低Cyclin D1、MMP2、MMP9蛋白表达水平(P<0.05),增加miR-497-5p表达水平。与miR-NC组相比,miR-497-5p组细胞活性、迁移率降低(P<0.05),克隆细胞数减少(P<0.05),Cyclin D1、MMP2、MMP9蛋白表达水平降低(P<0.05)。抑制miR-497-5p可以逆转补骨脂异黄酮对瘢痕疙瘩成纤维细胞增殖、迁移的影响。结论:补骨脂异黄酮可能通过上调miR-497-5p抑制瘢痕疙瘩成纤维细胞增殖和迁移。Objective To investigate the mechanism of psoralen isoflavones inhibiting the proliferation and migration of keloid fibroblasts by regulating miR-497-5p.Methods Keloid fibroblasts were cultured in vitro and treated with 0,9,18,36μg/ml psoralen isoflavones,which were classified as Con group,low dose group,medium dose group and high dose group.The cells were transfected with miR-NC and miR-497-5p by liposome method,and were denoted as miR-NC group and miR-497-5p group.After transfection with anti-miR-NC and anti-miR-497-5p by liposome method,the cells were treated with 36μg/ml psoralen isoflavones,which were denoted as high-dose group+anti-miR-NC group and high-dose group+anti-miR-497-5p group.Cell proliferation was detected by CCK8 and cloning assay.Cell migration was detected by wound healing test.The expression levels of Cyclin D1,MMP2 and MMP9 were detected by Western blot assay.The expression level of miR-497-5p was detected by qRT-PCR.Results Psoralen isoflavones decreased the cell viability and migration rate of keloidfibroblasts(P<0.05),decreased the number of cloned cells(P<0.05),and decreased the protein expression levels of Cyclin D1,MMP2,and MMP9(P<0.05),increase the expression level of miR-497-5p.Compared with the miR-NC group,the cell viability and migration rate of the miR-497-5p group were reduced(P<0.05),the number of cloned cells was reduced(P<0.05),and the protein expression levels of Cyclin D1,MMP2,and MMP9 were reduced(P<0.05).Inhibition of miR-497-5p can reverse the effect of psoralen isoflavones on the proliferation and migration of keloidfibroblasts.Conclusion Psoralen isoflavones may inhibit the proliferation and migration of keloidfibroblasts by increasing miR-497-5p.

关 键 词：补骨脂异黄酮 miR-497-5p 瘢痕疙瘩成纤维细胞 增殖 迁移 

分 类 号：R285[医药卫生—中药学]