黄秋葵多糖和黄酮的双水相萃取  

Study on the aqueous two-phase extraction of polysaccharides and flavonoids from okra

作　　者：郭晨忆 李帆[1] 窦啸天 张宜兴 李佳雪 祝子坪[1] Guo Chenyi;Li Fan;Dou Xiaotian;Zhang Yixing;Li Jiaxue;Zhu Ziping(School of Pharmaceutical Sciences,Taizhou University,Taizhou 318000,China)

机构地区：[1]台州学院药学院,浙江台州318000

出　　处：《台州学院学报》2024年第3期29-35,共7页Journal of Taizhou University

基　　金：国家级大学生创新创业训练计划项目(202310350068);台州市科技计划项目(21nya18)。

摘　　要：为了从黄秋葵中分离出多糖和黄酮,采用(NH_(4))_(2)SO_(4)和乙醇形成的双水相进行萃取,经单因素和正交试验优化萃取条件。结果表明:每10 mL萃取液含1.98 g(NH_(4))_(2)SO_(4)和3.9 mL乙醇,50℃条件下振荡萃取15 min,黄秋葵多糖萃取率为(86.12±1.87)%(n=3),黄酮萃取率为(78.38±1.35)%(n=3)。该方法可以一次性萃取出黄秋葵多糖和黄酮,且萃取率较高,为黄秋葵功能性成分的提取奠定了基础。To separate polysaccharides and flavonoids from okra,an aqueous two-phase composed of(NH_(4))_(2)SO_(4) and ethanol was used for the extraction.The extraction conditions were optimized through single-factor and orthogonal tests.The results showed that under the conditions that for each 10 mL of the extract containing 1.98 g(NH_(4))_(2)SO_(4) and 3.9 mL ethanol,extracted at 50°C for 15 minutes,the extraction rate in okra was(86.12±1.87)%(n=3)for polysaccharide and was(78.38±1.35)%(n=3)for flavonoids.This method can extract okra polysaccharides and flavonoids at one time,with a high extraction rate,and can lay the foundation for the extraction of functional components of okra.

关 键 词：黄秋葵 多糖 黄酮 双水相萃取 

分 类 号：S64[农业科学—蔬菜学]